FACS analysis of the planarian stem cell compartment as a tool to understand regenerative mechanisms.

FACS analysis of the planarian stem cell compartment as a tool to understand regenerative mechanisms.
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对涡虫干细胞室进行 FACS 分析作为了解再生机制的工具。

DOI:
10.1007/978-1-61779-980-8_13
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发表时间:
2012
影响因子:
--
通讯作者:
A. Aboobaker
A. Aboobaker
中科院分区:
--
文献类型:
--
作者:
B. T. Romero;Deborah J. Evans;A. Aboobaker

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行星动物提供了一个相对简单的模型系统来研究干细胞动力学和再生现象。与其他系统一样,了解干细胞和干细胞后代的动力学对于了解协调干细胞生物学的分子机制至关重要。浮游生物拥有丰富的成体干细胞群,这可以通过荧光激活细胞分选(FACS)观察到。这种方法允许对不同的干细胞亚群及其后代进行监测和分类,以用于下游研究,以应对不同的再生场景、药物治疗或再生事件所需基因的RNAi敲除。
Planarians provide a relatively simple model system in which to study stem cell dynamics and regenerative phenomena. As with other systems understanding the dynamics of stem cell and stem cell progeny is crucial in order to get at the molecular mechanisms orchestrating stem cell biology. Planarians have an abundant adult stem cell population that can be observed using Fluorescence-Activated Cell Sorting (FACS). This approach allows different subpopulations of stem cells and their progeny to be monitored and sorted for downstream studies in response to different regenerative scenarios, drug treatments, or RNAi knockdown of genes required for regenerative events.
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