Planarian regeneration involves distinct stem cell responses to wounds and tissue absence.
Planarian regeneration involves distinct stem cell responses to wounds and tissue absence.
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DOI：
10.1016/j.ydbio.2010.06.017
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发表时间：
2010-08-15
影响因子：
2.7
通讯作者：
Reddien PW
中科院分区：
文献类型：
--
作者：
Wenemoser D;Reddien PW
再生需要来自伤口部位的用于检测伤口的信号，以及确定损伤的性质以指定适当的再生反应的机制。引起再生的伤口信号和组织对伤口的反应仍然知之甚少。Planarians能够从基本上任何类型的损伤中再生，并为研究再生启动中的伤口反应提供了一种新的系统。新开发的分子和细胞工具，现在可以使用的研究再生启动的Planarian Schmidtea mediterranea。Planarian再生需要称为neoblast的成体干细胞，截肢在再生早期触发了neoblast有丝分裂的两个高峰。我们证明，第一个有丝分裂高峰是一个全身性的反应，任何损伤，第二个，局部，neoblast反应诱导只有当损伤导致缺失的组织。第二种反应的特征是新生细胞向伤口募集，即使在完整动物中缺乏新生细胞的区域也是如此。随后，这些新成细胞被诱导在伤口附近分裂和分化，导致新组织的形成。我们的结论是，存在两个功能不同的信号阶段的干细胞伤口反应，区分简单的损伤和情况下，需要再生缺失的组织。
Regeneration requires signaling from a wound site for detection of the wound, and a mechanism that determines the nature of the injury to specify the appropriate regenerative response. Wound signals and tissue responses to wounds that elicit regeneration remain poorly understood. Planarians are able to regenerate from essentially any type of injury and present a novel system for the study of wound responses in regeneration initiation. Newly developed molecular and cellular tools now enable study of regeneration initiation using the planarian Schmidtea mediterranea. Planarian regeneration requires adult stem cells called neoblasts and amputation triggers two peaks in neoblast mitoses early in regeneration. We demonstrate that the first mitotic peak is a body-wide response to any injury and that a second, local, neoblast response is induced only when injury results in missing tissue. This second response was characterized by recruitment of neoblasts to wounds, even in areas that lack neoblasts in the intact animal. Subsequently, these neoblasts were induced to divide and differentiate near the wound, leading to formation of new tissue. We conclude that there exist two functionally distinct signaling phases of the stem cell wound response that distinguish between simple injury and situations that require the regeneration of missing tissue.
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影响因子：
--
作者：
Egger B;Gschwentner R;Hess MW;Nimeth KT;Adamski Z;Willems M;Rieger R;Salvenmoser W
通讯作者：
Salvenmoser W
影响因子：
64.8
作者：
LEE, MG;NORBURY, CJ;NURSE, P
通讯作者：
NURSE, P
影响因子：
--
作者：
BAGUNA, J
通讯作者：
BAGUNA, J
影响因子：
--
作者：
BEST, JB;HAND, S;ROSENVOLD, R
通讯作者：
ROSENVOLD, R
DOI：
10.1083/jcb.200509011
发表时间：
2006-01-30
期刊：
The Journal of cell biology
影响因子：
--
作者：
Morrison JI;Lööf S;He P;Simon A
通讯作者：
Simon A