Two Chikungunya isolates from the outbreak of La Reunion (Indian Ocean) exhibit different patterns of infection in the mosquito, Aedes albopictus.
Two Chikungunya isolates from the outbreak of La Reunion (Indian Ocean) exhibit different patterns of infection in the mosquito, Aedes albopictus.
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DOI：
10.1371/journal.pone.0001168
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发表时间：
2007-11-14
期刊：
影响因子：
3.7
通讯作者：
Failloux, Anna-Bella
中科院分区：
文献类型：
--
作者：
Vazeille, Marie;Moutailler, Sara;Coudrier, Daniel;Rousseaux, Claudine;Khun, Huot;Huerre, Michel;Thiria, Julien;Dehecq, Jean-Sebastien;Fontenille, Didier;Schuffenecker, Isabelle;Despres, Philippe;Failloux, Anna-Bella
2005-2006年，留尼汪岛爆发了基孔肯雅(Chik)疫情。涉案媒介为白纹伊蚊。在这里，我们提出了第一个关于Ae易感性的研究。将白纹伊蚊种群与来自留尼汪岛的同域CHIKV分离株进行比较，并将其与其他病毒/媒介组合进行比较。我们口服感染了8Ae。2006年3月从留尼汪岛采集的白纹伊蚊和从马约特采集的3株白纹伊蚊和来自留尼汪岛的两种基孔肯雅病毒：(I)2005年6月收集的05.115株，其糖蛋白E1的第226位有一个丙氨酸；(Ii)2005年11月收集的06.21号株，替代了A226V。另外两个CHIKV分离株和另外四个蚊子品系/物种也进行了测试。感染血粉病毒滴度为107个空斑形成单位(PFU)/毫升。在感染14天后，通过免疫荧光染色对存活的雌性南瓜的头部进行播散率评估。与CHIKV 05.115相比，CHIKV06.21的发病率至少高出两倍。另外，每天对10例个体进行定量RT-PCR分析。病毒RNA在(I)整个雌性和(Ii)感染雌性的中肠和唾液腺上进行了定量。在比较图谱时，CHIKV06.21产生的病毒RNA拷贝比CHIKV06.21多近2logRNA拷贝。此外，感染05.115新城疫病毒的女性可分为两类：弱易感和强易感，与感染新城疫病毒06.21的人相当。组织学分析发现在感染后两天唾液腺中存在CHIKV。此外，Ae.留尼汪岛的白纹伊蚊是与Ae一样有效的媒介。Aegypti和Ae.越南白纹伊蚊感染CHIKV 06.21。我们的发现支持这样一种假设，即留尼汪岛的Chik疫情是由于一种高能力的媒介Ae所致。白纹伊蚊，使CHIKV 06.21得以有效复制和传播。
A Chikungunya (CHIK) outbreak hit La Réunion Island in 2005–2006. The implicated vector was Aedes albopictus. Here, we present the first study on the susceptibility of Ae. albopictus populations to sympatric CHIKV isolates from La Réunion Island and compare it to other virus/vector combinations. We orally infected 8 Ae. albopictus collections from La Réunion and 3 from Mayotte collected in March 2006 with two Chikungunya virus (CHIKV) from La Réunion: (i) strain 05.115 collected in June 2005 with an Alanine at the position 226 of the glycoprotein E1 and (ii) strain 06.21 collected in November 2005 with a substitution A226V. Two other CHIKV isolates and four additional mosquito strains/species were also tested. The viral titer of the infectious blood-meal was 107 plaque forming units (pfu)/mL. Dissemination rates were assessed by immunofluorescent staining on head squashes of surviving females 14 days after infection. Rates were at least two times higher with CHIKV 06.21 compared to CHIKV 05.115. In addition, 10 individuals were analyzed every day by quantitative RT-PCR. Viral RNA was quantified on (i) whole females and (ii) midguts and salivary glands of infected females. When comparing profiles, CHIKV 06.21 produced nearly 2 log more viral RNA copies than CHIKV 05.115. Furthermore, females infected with CHIKV 05.115 could be divided in two categories: weakly susceptible or strongly susceptible, comparable to those infected by CHIKV 06.21. Histological analysis detected the presence of CHIKV in salivary glands two days after infection. In addition, Ae. albopictus from La Réunion was as efficient vector as Ae. aegypti and Ae. albopictus from Vietnam when infected with the CHIKV 06.21. Our findings support the hypothesis that the CHIK outbreak in La Réunion Island was due to a highly competent vector Ae. albopictus which allowed an efficient replication and dissemination of CHIKV 06.21.
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DOI：
10.1016/j.trstmh.2004.03.013
发表时间：
2005-02-01
影响因子：
2.2
作者：
Laras, K;Sukri, NC;Corwin, AL
通讯作者：
Corwin, AL
影响因子：
5.4
作者：
Ahn, A;Gibbons, DL;Kielian, M
通讯作者：
Kielian, M
影响因子：
2.1
作者：
KONISHI, E;YAMANISHI, H
通讯作者：
YAMANISHI, H
影响因子：
3.8
作者：
Khan, AH;Morita, K;Igarashi, A
通讯作者：
Igarashi, A
中国按蚊群（双翅目：蚊科）：核糖体 DNA 内转录间隔区 2 序列推断的物种区分和系统发育关系
影响因子：
2.1
作者：
Afrane, YA;Lawson, BW;Yan, GY
通讯作者：
Yan, GY