Transcriptome analysis of functional differentiation between haploid and diploid cells of Emiliania huxleyi, a globally significant photosynthetic calcifying cell.
Transcriptome analysis of functional differentiation between haploid and diploid cells of Emiliania huxleyi, a globally significant photosynthetic calcifying cell.
复制标题
DOI：
10.1186/gb-2009-10-10-r114
复制
发表时间：
2009
期刊：
影响因子：
12.3
通讯作者：
de Vargas C
中科院分区：
文献类型：
--
作者：
von Dassow P;Ogata H;Probert I;Wincker P;Da Silva C;Audic S;Claverie JM;de Vargas C
对浮游植物赫氏艾米利亚的EST分析揭示了参与单倍体和二倍体特异性过程的基因，并为环境适应提供了见解。真核生物分为单倍体、双倍体或单倍-双倍体，这取决于在生命周期中进行有丝分裂的倍性水平。赫氏艾米利亚藻是海洋中最丰富的浮游植物之一，在全球碳通量中发挥着重要作用，并代表着一种在真核生物进化早期分化的神秘单细胞生物。本种为单倍-双倍型。对单倍体细胞知之甚少，但有人假设它们允许物种在二倍体细胞每年开花之间持续存在。我们对来自单倍体和二倍体E. huxleyi将cDNA文库标准化，以鉴定参与每个生命阶段（2N钙化或1 N运动）的重要过程的基因，并更好地了解这种突出的单倍-二倍体生物体的单倍体阶段。单倍体和二倍体转录组显示出显著的分化，二倍体细胞中的转录组丰富度比单倍体细胞高约20%，并且估计两个阶段之间只有≤ 50%的转录物是共同的。分化单倍体的转录本的主要功能类别包括信号传导和运动基因。二倍体特异性转录物包括Ca 2+、H+和HCO 3-泵。转录组的潜在差异因素包括单倍体特异性Myb转录因子同源物和一个不寻常的二倍体特异性组蛋白H4同源物。这项研究允许识别可能参与二倍体特异性生物矿化，单倍体特异性运动和转录控制的基因。二倍体细胞中更大的转录组丰富性表明它们可能更通用于利用丰富环境的多样性，而单倍体细胞本质上更流线型。
An EST analysis of the phytoplankton Emiliania huxleyi reveals genes involved in haploid- and diploid-specific processes and provides insights into environmental adaptation. Eukaryotes are classified as either haplontic, diplontic, or haplo-diplontic, depending on which ploidy levels undergo mitotic cell division in the life cycle. Emiliania huxleyi is one of the most abundant phytoplankton species in the ocean, playing an important role in global carbon fluxes, and represents haptophytes, an enigmatic group of unicellular organisms that diverged early in eukaryotic evolution. This species is haplo-diplontic. Little is known about the haploid cells, but they have been hypothesized to allow persistence of the species between the yearly blooms of diploid cells. We sequenced over 38,000 expressed sequence tags from haploid and diploid E. huxleyi normalized cDNA libraries to identify genes involved in important processes specific to each life phase (2N calcification or 1N motility), and to better understand the haploid phase of this prominent haplo-diplontic organism. The haploid and diploid transcriptomes showed a dramatic differentiation, with approximately 20% greater transcriptome richness in diploid cells than in haploid cells and only ≤ 50% of transcripts estimated to be common between the two phases. The major functional category of transcripts differentiating haploids included signal transduction and motility genes. Diploid-specific transcripts included Ca2+, H+, and HCO3- pumps. Potential factors differentiating the transcriptomes included haploid-specific Myb transcription factor homologs and an unusual diploid-specific histone H4 homolog. This study permitted the identification of genes likely involved in diploid-specific biomineralization, haploid-specific motility, and transcriptional control. Greater transcriptome richness in diploid cells suggests they may be more versatile for exploiting a diversity of rich environments whereas haploid cells are intrinsically more streamlined.
登录
查看更多内容
影响因子：
14.9
作者：
UniProt Consortium
通讯作者：
UniProt Consortium
影响因子：
2.9
作者：
Araki, Y;Gonzalez, EL
通讯作者：
Gonzalez, EL
影响因子：
3.5
作者：
Bhan, Sheetal;May, Warren;Sittman, Donald B.
通讯作者：
Sittman, Donald B.
DOI：
10.1099/00207713-52-2-297
发表时间：
2002-03-01
影响因子：
2.8
作者：
Cavalier-Smith, T
通讯作者：
Cavalier-Smith, T
影响因子：
64.8
作者：
BINDER, BJ;ANDERSON, DM
通讯作者：
ANDERSON, DM