3D Microperiodic Hydrogel Scaffolds for Robust Neuronal Cultures.
3D Microperiodic Hydrogel Scaffolds for Robust Neuronal Cultures.
复制标题
DOI：
10.1002/adfm.201001746
复制
发表时间：
2011-01-07
影响因子：
19
通讯作者：
Nuzzo, Ralph G.
中科院分区：
文献类型：
--
作者：
Shepherd, Jennifer N. Hanson;Parker, Sara T.;Shepherd, Robert F.;Gillette, Martha U.;Lewis, Jennifer A.;Nuzzo, Ralph G.
采用可光聚合水凝胶墨水的直写组装方法制备了聚甲基丙烯酸羟乙酯(PHEMA)三维微周期支架。该油墨最初由物理缠绕的PHEMA链组成，溶解在HEMA单体、共聚单体、光引发剂和水的溶液中。在打印不同结构的3D支架时，墨丝暴露在紫外光下，在那里它们被转化为由化学交联和物理缠绕的PHEMA链组成的相互穿透的水凝胶网络。通过对多赖氨酸的吸收，这些三维微周期支架对原代培养的大鼠海马神经元具有生长顺应性。神经细胞在这些支架上茁壮成长，形成分化的、错综复杂的分支网络。激光共聚焦扫描显微镜显示，细胞的分布和神经元突起排列的程度取决于支架的结构。这项工作为建立适合体外研究敏感细胞类型的平台向前迈出了重要的一步。
Three-dimensional (3D) microperiodic scaffolds of poly(2-hydroxyethyl methacrylate) (pHEMA) have been fabricated by direct-write assembly of a photopolymerizable hydrogel ink. The ink is initially composed of physically entangled pHEMA chains dissolved in a solution of HEMA monomer, comonomer, photoinitiator and water. Upon printing 3D scaffolds of varying architecture, the ink filaments are exposed to UV light, where they are transformed into an interpenetrating hydrogel network of chemically cross-linked and physically entangled pHEMA chains. These 3D microperiodic scaffolds are rendered growth compliant for primary rat hippocampal neurons by absorption of polylysine. Neuronal cells thrive on these scaffolds, forming differentiated, intricately branched networks. Confocal laser scanning microscopy reveals that both cell distribution and extent of neuronal process alignment depend upon scaffold architecture. This work provides an important step forward in the creation of suitable platforms for in vitro study of sensitive cell types.
登录
查看更多内容
影响因子：
14
作者：
Willerth, Stephanie M.;Arendas, Kelly J.;Sakiyama-Elbert, Shelly Elese
通讯作者：
Sakiyama-Elbert, Shelly Elese
影响因子：
41.2
作者：
Luo, Y;Shoichet, MS
通讯作者：
Shoichet, MS
影响因子：
56.9
作者：
Cukierman, E;Pankov, R;Yamada, KM
通讯作者：
Yamada, KM
DOI：
10.1073/pnas.97.12.6728
发表时间：
2000-06-06
影响因子：
11.1
作者：
Holmes, TC;de Lacalle, S;Zhang, SG
通讯作者：
Zhang, SG
影响因子：
56.9
作者：
LANGER, R;VACANTI, JP
通讯作者：
VACANTI, JP