Enhanced biosynthesis of withanolides by elicitation and precursor feeding in cell suspension culture of Withania somnifera (L.) Dunal in shake-flask culture and bioreactor.
Enhanced biosynthesis of withanolides by elicitation and precursor feeding in cell suspension culture of Withania somnifera (L.) Dunal in shake-flask culture and bioreactor.
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DOI：
10.1371/journal.pone.0104005
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Manickavasagam M
中科院分区：
文献类型：
--
作者：
Sivanandhan G;Selvaraj N;Ganapathi A;Manickavasagam M
本研究采用诱导法和前体补料法，采用摇瓶培养和生物反应器技术，研究了睡茄悬浮细胞中主要和次要睡茄内酯的生物合成。激发剂，如氯化镉，氯化铝和壳聚糖，前体，如胆固醇，甲羟戊酸和角鲨烯进行了检查。检出的最大睡茄内酯总量[睡茄内酯A（7606.75 mg），含阿托咪B（4826.05 mg），醉茄素A（3732.81 mg），丙酮（6538.65 mg），12脱氧withanstramonolide（3176.63 mg），睡茄苷IV（2623.21 mg）和睡茄苷V（2861.18 mg（100 mg/l）和角鲨烯（6 mM）沿着1 mg/l毒莠定、0.5 mg/l KN、200 mg/l L-谷氨酰胺和5%蔗糖一起在生物反应器中培养的第28天分别暴露4 h和48 h。与对照处理相比，我们在摇瓶培养（2.13倍）以及生物反应器（1.66倍）中获得了更高浓度的总睡茄内酯。该优化方案可用于工业生物反应器在短培养时间内从悬浮培养中生产睡茄内酯的商业化水平。
The present study investigated the biosynthesis of major and minor withanolides of Withania somnifera in cell suspension culture using shake-flask culture and bioreactor by exploiting elicitation and precursor feeding strategies. Elicitors like cadmium chloride, aluminium chloride and chitosan, precursors such as cholesterol, mevalonic acid and squalene were examined. Maximum total withanolides detected [withanolide A (7606.75 mg), withanolide B (4826.05 mg), withaferin A (3732.81 mg), withanone (6538.65 mg), 12 deoxy withanstramonolide (3176.63 mg), withanoside IV (2623.21 mg) and withanoside V (2861.18 mg)] were achieved in the combined treatment of chitosan (100 mg/l) and squalene (6 mM) along with 1 mg/l picloram, 0.5 mg/l KN, 200 mg/l L-glutamine and 5% sucrose in culture at 4 h and 48 h exposure times respectively on 28th day of culture in bioreactor. We obtained higher concentrations of total withanolides in shake-flask culture (2.13-fold) as well as bioreactor (1.66-fold) when compared to control treatments. This optimized protocol can be utilized for commercial level production of withanolides from suspension culture using industrial bioreactors in a short culture period.
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影响因子：
2.9
作者：
Mishra, Bhawana;Sangwan, Rajender S.;Sangwan, Neelam S.
通讯作者：
Sangwan, Neelam S.
影响因子：
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MURASHIGE, T;SKOOG, F
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SKOOG, F