A study of gluco-corticosteroid-induced pyknosis in the thymus and lymph node of the adrenalectomized rat.
A study of gluco-corticosteroid-induced pyknosis in the thymus and lymph node of the adrenalectomized rat.
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DOI：
10.1083/jcb.50.3.583
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发表时间：
1971-09
期刊：
影响因子：
--
通讯作者：
de Harven E
中科院分区：
文献类型：
--
作者：
La Pushin RW;de Harven E
在类固醇给药大鼠胸腺中观察到的固缩核致密、均匀、强嗜碱性和Feulgen阳性。在电子显微镜下，染色质从核液中完全分离，产生边缘或新月状的浓缩染色质。大约30%的所有小胸腺细胞似乎在给予合成皮质类固醇地塞米松后3-4小时内经历这种类型的变性。此时，观察到成簇的固缩胸腺细胞，可能是致密网状细胞和巨噬细胞活动的结果。地形和实验数据表明，存在一个选定的类固醇敏感的胸腺细胞群体。此外，胸苷-3H掺入研究的基础上，似乎类固醇敏感的胸腺细胞群体不对应于“老化”细胞。此外，许多浆细胞在相同的类固醇治疗后变得固缩，表明它们的细胞核与淋巴细胞的细胞核之间存在意想不到的相似性。最后，在各种体外实验中，类固醇未能诱导胸腺细胞固缩，表明类固醇的体内作用是间接的。结果进行了讨论（a）核固缩特征的变化的性质，（B）类固醇触发这种变化的假设机制，和（c）人口的细胞容易类固醇诱导的固缩。
Pyknotic nuclei, observed in the thymus of steroid-treated rats, are dense, homogeneous, intensely basophilic and Feulgen positive. Under the electron microscope, the image is that of a complete segregation of the chromatin from the nuclear sap producing a margin or crescent of condensed chromatin. Approximately 30% of all small thymocytes appeared to undergo this type of degeneration within 3–4 hr after administration of the synthetic corticosteroid, dexamethasone. At this time, pyknotic thymocytes were observed in clusters, probably as a result of the activity of dense reticular cells and macrophages. Topographical and experimental data suggest the existence of a select population of steroid-sensitive thymic cells. Furthermore, on the basis of thymidine-3H incorporation studies, it appears that the steroid-sensitive population of thymocytes does not correspond to "aged" cells. In addition, many plasma cells became pyknotic after the same steroid treatment, indicating an unexpected similarity between their nuclei and those of lymphocytes. Finally, steroid failed to induce pyknosis of thymocytes in a variety of in vitro experiments, suggesting that the in vivo effect of steroid is of an indirect nature. The results are discussed in terms of (a) the nature of the nuclear changes characterizing pyknosis, (b) the hypothetical mechanism whereby steroids trigger such changes, and (c) the population of cells susceptible to steroid-induced pyknosis.
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影响因子：
7.8
作者：
CARO, LG;KOLB, JA;VANTUBERGEN, RP
通讯作者：
VANTUBERGEN, RP
DOI：
10.1084/jem.120.3.389
发表时间：
1964-09-01
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
CRADDOCK CG;NAKAI GS;FUKUTA H;VANSLAGER LM
通讯作者：
VANSLAGER LM
DOI：
10.1111/j.1749-6632.1964.tb40692.x
发表时间：
1964-01-01
影响因子：
5.2
作者：
FLIEDNER, TM;ROBERTSON, JS;KESSE, M
通讯作者：
KESSE, M
影响因子：
64.8
作者：
HILL, M
通讯作者：
HILL, M
DOI：
10.1016/s0074-7696(08)61836-7
发表时间：
1967-01-01
期刊：
International Review of Cytology
影响因子：
--
作者：
Everett, N. B.;Tyler, R. W.
通讯作者：
Tyler, R. W.