Competition and complementation between thymidine kinase-negative and wild-type herpes simplex virus during co-infection of mouse trigeminal ganglia.
Competition and complementation between thymidine kinase-negative and wild-type herpes simplex virus during co-infection of mouse trigeminal ganglia.
复制标题
胸苷激酶阴性和野生型单纯疱疹病毒在小鼠三叉神经节共感染过程中的竞争和互补。
作者：
Shih;Yu;A. Griffiths;Wen;Shun
缺乏胸苷激酶（TK）的单纯疱疹病毒实验室株不能在小鼠三叉神经节中快速复制到可检测的水平，也不能从潜伏期重新激活。然而，许多对抗病毒药物阿昔洛韦耐药的致病性临床分离株是TK阴性（TK（-））和TK阳性（TK（+））病毒的异质群体。为了在体内概括这一点，用野生型病毒和重组TK（-）突变体以不同比例的混合物感染小鼠。合并感染后，三叉神经节中TK（+）病毒的复制、潜伏病毒基因组数量和再活化效率均以与接种物中TK（-）病毒量相关的方式降低。TK（+）病毒并不总是补充小鼠神经节内TK（-）突变体的急性复制或增加潜伏病毒基因组的数量。即便如此，TK（+）病毒仍然可以将致病性表型赋予TK（-）突变体，以某种方式提供足够的反式TK活性，使TK（-）突变体能够从潜伏感染的神经节中重新激活。
Laboratory strains of herpes simplex virus lacking thymidine kinase (TK) cannot replicate acutely to detectable levels in mouse trigeminal ganglia and do not reactivate from latency. However, many pathogenic clinical isolates that are resistant to the antiviral drug acyclovir are heterogeneous populations of TK-negative (TK(-)) and TK-positive (TK(+)) viruses. To recapitulate this in vivo, mice were infected with mixtures of wild-type virus and a recombinant TK(-) mutant in various ratios. Following co-infection, the replication, number of latent viral genomes and reactivation efficiency of TK(+) virus in trigeminal ganglia were reduced in a manner related to the amount of TK(-) virus in the inoculum. TK(+) virus did not always complement the acute replication or increase the number of latent viral genomes of TK(-) mutant in mouse ganglia. Even so, TK(+) virus could still confer the pathogenic phenotype to a TK(-) mutant, somehow providing sufficient TK activity in trans to permit a TK(-) mutant to reactivate from latently infected ganglia.
影响因子：
3.7
作者：
Jacobson, JG;Chen, SH;Coen, DM
通讯作者：
Coen, DM
DOI：
10.1073/pnas.86.12.4736
发表时间：
1989-06-01
影响因子：
11.1
作者：
COEN, DM;KOSZVNENCHAK, M;KNIPE, DM
通讯作者：
KNIPE, DM
HSV-1 温度敏感突变体的遗传分析：结合使用互补和物理作图进行顺反子分配。
影响因子：
3.7
作者：
Weller,SK;Aschman,DP;Sacks,WR;Coen,DM;Schaffer,PA
通讯作者：
Schaffer,PA