Nonlinear material and ionic transport through membrane nanotubes.
Nonlinear material and ionic transport through membrane nanotubes.
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DOI：
10.1016/j.bbamem.2021.183677
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发表时间：
2021-10-01
期刊：
影响因子：
--
通讯作者：
Frolov VA
中科院分区：
文献类型：
--
作者：
Ivchenkov DV;Kuzmin PI;Galimzyanov TR;Shnyrova AV;Bashkirov PV;Frolov VA
膜纳米管（NTs）及其网络在细胞膜运输和细胞间通讯中起着重要作用。NT腔的传输特性类似于常规固态纳米孔的传输特性。然而，与刚性孔不同，NT的软膜壁可以通过驱动通过NT管腔的运输的力而变形。NT几何形状和传输特性之间的这种内在耦合仍然很少探索。使用同步荧光显微镜和电导测量，我们发现NT形状因电场力和流体静力驱动离子和溶质通量通过NT腔而发生变化。远离形状不稳定性，力效应的强度由横向膜张力确定，并且与膜弹性成比例，使得NT可以作为线性弹性传感器操作。接近形状不稳定性，运输力引发大规模的形状变换，随机和周期性的。周期性振荡耦合到囊泡通道沿着NT轴，类似蠕动运输。振荡由电场参数控制，使NT成为具有生物和技术意义的高度非线性纳米流体电路元件。
Membrane nanotubes (NTs) and their networks play an important role in intracellular membrane transport and intercellular communications. The transport characteristics of the NT lumen resemble those of conventional solid-state nanopores. However, unlike the rigid pores, the soft membrane wall of the NT can be deformed by forces driving the transport through the NT lumen. This intrinsic coupling between the NT geometry and transport properties remains poorly explored. Using synchronized fluorescence microscopy and conductance measurements, we revealed that the NT shape was changed by both electric and hydrostatic forces driving the ionic and solute fluxes through the NT lumen. Far from the shape instability, the strength of the force effect is determined by the lateral membrane tension and is scaled with membrane elasticity so that the NT can be operated as a linear elastic sensor. Near shape instabilities, the transport forces triggered large-scale shape transformations, both stochastic and periodic. The periodic oscillations were coupled to a vesicle passage along the NT axis, resembling peristaltic transport. The oscillations were parametrically controlled by the electric field, making NT a highly nonlinear nanofluidic circuitry element with biological and technological implications.
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DOI：
10.1134/s1990747807020110
发表时间：
2007-06-01
影响因子：
0.5
作者：
Bashkirov, P. V.
通讯作者：
Bashkirov, P. V.
影响因子：
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作者：
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影响因子：
14.8
作者：
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通讯作者：
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影响因子：
17.4
作者：
Kozak D;Anderson W;Vogel R;Trau M
通讯作者：
Trau M
影响因子：
2.4
作者：
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