Whole-Transcriptome Analysis Reveals Long Noncoding RNAs Involved in Female Floral Development of Hickory (Carya cathayensis Sarg.).
Whole-Transcriptome Analysis Reveals Long Noncoding RNAs Involved in Female Floral Development of Hickory (Carya cathayensis Sarg.).
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全转录组分析显示，山核桃女性花卉发育（Carya Cathayensis sarg。）涉及长期非编码RNA。
DOI：
10.3389/fgene.2022.910488
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发表时间：
2022
影响因子：
3.7
通讯作者：
中科院分区：
文献类型：
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作者：
山核桃是我国特有的木本油料和果树树种，具有很高的经济价值。然而，山核桃幼年期长，雌雄花期不一致，影响了坐果率，进一步限制了果实产量。目前，据报道，长非编码RNA（lncRNA）在生物过程中发挥着关键的调节作用。然而，lncRNA 在山核桃雌花发育中的作用仍不清楚。在这项研究中，从山核桃三个不同生长阶段的雌花转录组中总共鉴定出了 6,862 个假定的 lncRNA。根据京都基因与基因组百科全书（KEGG）的富集结果，我们提出lncRNA可能在花形成的植物激素信号传导过程中发挥重要作用，特别是在脱落酸和茉莉酸途径中。此外，我们预测了四种 microRNA (miRNA)、三种 lncRNA 和四种基因之间的相互作用。我们提出，面对不断变化的环境，LNC_002115 与 PHOSPHATE2 (PHO2) 竞争 cca-miR399f 上的结合位点，并保护 PHO2 免受抑制。此外，顺式作用 LNC_002115 通过影响 ABRE 结合因子 (ABF) 来调节短营养期 (SVP) 的表达。简而言之，LNC_002115 通过影响 PHO2 和 SVP 来调节山核桃雌性花的发育。这项研究首次鉴定了参与山核桃雌花发育的lncRNA，并为阐明lncRNA及其靶标如何在山核桃雌花发育中发挥作用提供了新的见解，从而为进一步研究开花相关lncRNA的功能表征提供了机会。
Hickory, an endemic woody oil and fruit tree species in China, is of great economic value. However, hickory has a long juvenile period and an inconsistent flowering of males and females, thus influencing the bearing rates and further limiting fruits yield. Currently, it is reported that long noncoding RNAs (lncRNAs) play critical regulatory roles in biological processes. However, the role of lncRNAs in the development of hickory female flowers remains unclear. In this study, a total of 6,862 putative lncRNAs were identified from the female flower transcriptomes in three different growth stages of hickory. We proposed that lncRNAs might play an important role in phytohormone signaling processes for flower formation, especially in the abscisic acid and jasmonic acid pathways, according to the results of our Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment. Moreover, we predicted the interactions among four microRNAs (miRNAs), three lncRNAs, and four genes. We proposed that facing the changing environment, LNC_002115 competes with PHOSPHATE2 (PHO2) for the binding sites on cca-miR399f, and protects PHO2 from suppression. In addition, cis-acting LNC_002115 regulates the expression of the SHORT VEGETATIVE PHASE (SVP) by influencing ABRE-binding factor (ABF). In brief, LNC_002115 regulates hickory female floral development by impacting both PHO2 and SVP. This study was the first to identify lncRNAs involved in hickory female floral development, and provided new insight to elucidate how lncRNAs and their targets play a role in female floral development in hickory, thus unfolding the opportunities for functional characterization of blossom-related lncRNAs in further studies.
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