Identification of differentially expressed proteins in Ostrinia furnacalis adults after exposure to ultraviolet A
Identification of differentially expressed proteins in Ostrinia furnacalis adults after exposure to ultraviolet A
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紫外线A照射后亚洲玉米螟成虫差异表达蛋白的鉴定
DOI：
10.1007/s11356-018-2580-3
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发表时间：
2018-06
影响因子：
5.8
通讯作者：
Jianyu Meng
中科院分区：
文献类型：
--
作者：
Changyu Zhang;Jianyu Meng
紫外线A（UVA）是太阳紫外线辐射的主要成分，是一种重要的环境因子，可诱导昆虫细胞死亡、光感受器损伤和氧化应激。为了进一步了解昆虫对UVA辐射的适应机制，采用比较蛋白质组学方法研究了昆虫在UVA辐射下的差异蛋白表达。三日龄成年人用UVA处理1 h。利用双向电泳（2-DE）检测了对照和UVA处理的昆虫的总蛋白。2-DE分析表明，在O. UVA照射后，分别。通过质谱分析鉴定出30个差异表达蛋白。所鉴定的蛋白质参与多种生物学过程，如信号转导、运输处理、细胞应激、代谢和细胞骨架组织。我们的研究结果表明，O。对UVA辐射的适应反应是复杂的，并提供了新的见解UVA辐射胁迫。
Ultraviolet A (UVA), the major component of solar UV irradiation, is an important environmental factor inducing damage to insects including cell death, photoreceptor damage, and oxidative stress. In order to improve understanding of the adaptation mechanisms of insect after UVA exposure, a comparative proteomic analysis was carried out to reveal differential protein expression in Ostrinia furnacalis. Three-day-old adults were treated with UVA for 1 h. Total proteins of control and UVA-treated insects were examined using two-dimensional electrophoresis (2-DE). 2-DE analysis demonstrated that 19 proteins were increased and 18 proteins were decreased significantly in O. furnacalis after UVA exposure, respectively. Thirty differentially expressed proteins were successfully identified by mass spectrometry. The identified proteins were involved in diverse biological processes, such as signal transduction, transport processing, cellular stress, metabolisms, and cytoskeleton organization. Our results reveal that the response patterns of O. furnacalis to UVA irradiation are complex and provide novel insights into the adaptation response to UVA irradiation stress.
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影响因子：
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作者：
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作者：
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影响因子：
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