Requirement of CHROMOMETHYLASE3 for somatic inheritance of the spontaneous tomato epimutation Colourless non-ripening.
Requirement of CHROMOMETHYLASE3 for somatic inheritance of the spontaneous tomato epimutation Colourless non-ripening.
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自发性番茄表突变体细胞遗传需要甲基化酶 3 无色未成熟
DOI：
10.1038/srep09192
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发表时间：
2015-03-17
影响因子：
4.6
通讯作者：
Hong Y
中科院分区：
文献类型：
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作者：
Chen W;Kong J;Qin C;Yu S;Tan J;Chen YR;Wu C;Wang H;Shi Y;Li C;Li B;Zhang P;Wang Y;Lai T;Yu Z;Zhang X;Shi N;Wang H;Osman T;Liu Y;Manning K;Jackson S;Rolin D;Zhong S;Seymour GB;Gallusci P;Hong Y
自然产生的表位突变是罕见的，主要在植物中描述。然而，这些突变体如何保持其表观遗传标记以及它们是如何遗传的仍是未知的。在此，我们报道了在番茄中维持自发变异及其同源无色非成熟(Cnr)表型所需的ChROMOMETHYLASE 3(SlCMT3)和其他甲基转移酶。我们筛选了一系列与DNA甲基化相关的基因，可以拯救高甲基化的Cnr突变体。沉默发育调控的SlCMT3基因导致LeSPL-CNR表达增加，该基因编码位于CnR基因座的SBP-box转录因子，并触发CnR果实的正常成熟。其他关键成熟基因的表达也上调了。靶向测序和全基因组测序表明，CnR果实的成熟诱导与LeSPL-CNR启动子286-bp区域CHG位点的甲基化减少以及与LeMADS-RIN结合位点相关的差异甲基化区域的DNA甲基化减少有关。我们的结果表明，在CNR基因座上可能存在不同甲基转移酶的协同作用，而植物特有的SLCMT3是维持CNR表观等位基因所必需的。DNA甲基化动态的维持对于CnR突变体的体细胞稳定性和番茄未成熟表型的遗传至关重要。
Naturally-occurring epimutants are rare and have mainly been described in plants. However how these mutants maintain their epigenetic marks and how they are inherited remain unknown. Here we report that CHROMOMETHYLASE3 (SlCMT3) and other methyltransferases are required for maintenance of a spontaneous epimutation and its cognate Colourless non-ripening (Cnr) phenotype in tomato. We screened a series of DNA methylation-related genes that could rescue the hypermethylated Cnr mutant. Silencing of the developmentally-regulated SlCMT3 gene results in increased expression of LeSPL-CNR, the gene encodes the SBP-box transcription factor residing at the Cnr locus and triggers Cnr fruits to ripen normally. Expression of other key ripening-genes was also up-regulated. Targeted and whole-genome bisulfite sequencing showed that the induced ripening of Cnr fruits is associated with reduction of methylation at CHG sites in a 286-bp region of the LeSPL-CNR promoter and a decrease of DNA methylation in differentially-methylated regions associated with the LeMADS-RIN binding sites. Our results indicate that there is likely a concerted effect of different methyltransferases at the Cnr locus and the plant-specific SlCMT3 is essential for sustaining Cnr epi-allele. Maintenance of DNA methylation dynamics is critical for the somatic stability of Cnr epimutation and for the inheritance of tomato non-ripening phenotype.
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通过 SlymiR157 调节 LeSPL-CNR 表达影响番茄果实成熟
影响因子：
4.6
作者：
Chen W;Kong J;Lai T;Manning K;Wu C;Wang Y;Qin C;Li B;Yu Z;Zhang X;He M;Zhang P;Gu M;Yang X;Mahammed A;Li C;Osman T;Shi N;Wang H;Jackson S;Liu Y;Gallusci P;Hong Y
通讯作者：
Hong Y
影响因子：
9.2
作者：
Durand, Stephanie;Bouche, Nicolas;Camilleri, Christine
通讯作者：
Camilleri, Christine
影响因子：
64.8
作者：
Tarutani, Yoshiaki;Shiba, Hiroshi;Takayama, Seiji
通讯作者：
Takayama, Seiji
影响因子：
64.8
作者：
Jackson, JP;Lindroth, AM;Jacobsen, SE
通讯作者：
Jacobsen, SE
DOI：
10.1038/nrg2719
发表时间：
2010-03
期刊：
Nature reviews. Genetics
影响因子：
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作者：
通讯作者：
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