Tuning LeSPL-CNR expression by SlymiR157 affects tomato fruit ripening.
Tuning LeSPL-CNR expression by SlymiR157 affects tomato fruit ripening.
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通过 SlymiR157 调节 LeSPL-CNR 表达影响番茄果实成熟
DOI：
10.1038/srep07852
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发表时间：
2015-01-19
影响因子：
4.6
通讯作者：
Hong Y
中科院分区：
文献类型：
--
作者：
Chen W;Kong J;Lai T;Manning K;Wu C;Wang Y;Qin C;Li B;Yu Z;Zhang X;He M;Zhang P;Gu M;Yang X;Mahammed A;Li C;Osman T;Shi N;Wang H;Jackson S;Liu Y;Gallusci P;Hong Y
在植物中，microRNAs（miRNAs）在植物生长、发育、产量、胁迫反应以及与病原菌的相互作用中发挥着重要作用。然而，没有实验证明miRNA在功能上参与果实成熟，尽管许多miRNA已在果实中被分析。在这里，我们表明，SlymiR 157和SlymiR 156差异调节成熟和软化番茄（番茄）。SlymiR 157在正常番茄果实和无色非成熟（Cnr）表突变体中表达并发育调节。它通过miRNA诱导的mRNA降解和翻译抑制以可能的剂量依赖性方式调节关键成熟基因LeSPL-CNR的表达。前SlymiR 157或成熟SlymiR 157的病毒递送导致延迟成熟。此外，关键成熟调控基因的qRT-PCR分析表明，SlymiR 157靶点LeSPL-CNR可能影响LeMADS-RIN、LeHB 1、SlAP 2a和SlTAGL 1的表达。然而，SlymiR 156不影响成熟的开始，但它影响红熟期后的果实软化。我们的研究结果表明，与成熟网络的转录因子一起工作，SlymiR 157和SlymiR 156形成了一个关键的额外层的调节控制番茄果实成熟过程。
In plants, microRNAs (miRNAs) play essential roles in growth, development, yield, stress response and interactions with pathogens. However no miRNA has been experimentally documented to be functionally involved in fruit ripening although many miRNAs have been profiled in fruits. Here we show that SlymiR157 and SlymiR156 differentially modulate ripening and softening in tomato (Solanum lycopersicum). SlymiR157 is expressed and developmentally regulated in normal tomato fruits and in those of the Colourless non-ripening (Cnr) epimutant. It regulates expression of the key ripening gene LeSPL-CNR in a likely dose-dependent manner through miRNA-induced mRNA degradation and translation repression. Viral delivery of either pre-SlymiR157 or mature SlymiR157 results in delayed ripening. Furthermore, qRT-PCR profiling of key ripening regulatory genes indicates that the SlymiR157-target LeSPL-CNR may affect expression of LeMADS-RIN, LeHB1, SlAP2a and SlTAGL1. However SlymiR156 does not affect the onset of ripening, but it impacts fruit softening after the red ripe stage. Our findings reveal that working together with a ripening network of transcription factors, SlymiR157 and SlymiR156 form a critical additional layer of regulatory control over the fruit ripening process in tomato.
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