A novel implantable collagen gel assay for fibroblast traction and proliferation during wound healing.
A novel implantable collagen gel assay for fibroblast traction and proliferation during wound healing.
复制标题
一种用于伤口愈合过程中成纤维细胞牵引和增殖的新型植入式胶原凝胶测定。
DOI：
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复制
发表时间：
2002
影响因子：
2.2
通讯作者：
R. Tranquillo
中科院分区：
文献类型：
--
作者：
P. Enever;D. Shreiber;R. Tranquillo
背景
开发了一种用于研究伤口环境中细胞行为的新型植入式测定法。该试验的独特之处在于，它将体外试验的更多定量性质与体内伤口愈合模型的更大生理相关性相结合。
材料和方法
将细胞接种在生理学相关的生物基质中，胶原凝胶，包含在半渗透管中，然后在伤口愈合反应的不同阶段暴露于伤口环境的可溶性因子。在规定的时间点收获凝胶，并测量细胞增殖速率和凝胶压实。将这些数据与我们的细胞-基质机械相互作用理论相结合，以估计导致凝胶压实的细胞所施加的细胞牵引力。细胞形态和α-平滑肌肌动蛋白的表达也进行了表征。
结果
暴露于可溶性伤口环境的成纤维细胞的增殖和牵引力与在完全培养基中培养的类似胶原凝胶中的那些不同。在伤口愈合反应的过程中，增殖和牵引也有所不同。与第7 - 11天的伤口相比，第1 - 5天的伤口牵引力更高，增殖更低。与培养中的细胞相反，复苏的细胞不再对α-平滑肌肌动蛋白染色。
结论
随着伤口愈合反应的进行而发生的可溶性伤口环境的变化改变成纤维细胞牵引和迁移。我们已经开发了一种新的测定方法，该方法采用生理学相关的生物基质，并允许动态可溶性伤口环境对细胞牵引、增殖和其他现象（如蛋白质表达）的影响被量化。
BACKGROUND
A novel implantable assay for studying cellular behavior in the wound environment was developed. The assay is unique in that it combines the more quantitative nature of in vitro assays with the greater physiological relevance of in vivo wound healing models.
MATERIALS AND METHODS
Cells were seeded in a physiologically relevant biological matrix, a collagen gel, contained within a semipermeable tube, and then exposed to soluble factors of the wound environment at different stages of the wound healing response. Gels were harvested at prescribed time points, and cell proliferation rates and gel compaction were measured. These data were combined with our theory for cell-matrix mechanical interactions to estimate the cell traction exerted by the cells leading to gel compaction. Cell morphology and alpha-smooth muscle actin expression were also characterized.
RESULTS
The proliferation of and traction exerted by fibroblasts exposed to the soluble wound environment were different from those in similar collagen gels maintained in culture in complete medium. Proliferation and traction also varied over the course of the wound healing response. Traction was higher and proliferation lower in day 1-5 wounds compared to day 7-11 wounds. Recovered cells no longer stained for alpha-smooth muscle actin, in contrast to cells maintained in culture.
CONCLUSIONS
Changes in the soluble wound environment that occur as the wound healing response proceeds alter fibroblast traction and migration. We have developed a new assay that employs a physiologically relevant biological matrix and allows the effects of the dynamic soluble wound environment on cellular traction, proliferation, and other phenomena such as protein expression to be quantified.
DOI：
10.1115/1.2796072
发表时间：
1997-05-01
影响因子：
1.7
作者：
Barocas, VH;Tranquillo, RT
通讯作者：
Tranquillo, RT
DOI：
10.1115/1.429652
发表时间：
2000-06-01
影响因子：
1.7
作者：
Girton, TS;Oegema, TR;Tranquillo, RT
通讯作者：
Tranquillo, RT
影响因子：
15.9
作者：
MUSTOE, TA;PIERCE, GF;DEUEL, TF
通讯作者：
DEUEL, TF