Optogenetic recruitment of dorsal raphe serotonergic neurons acutely decreases mechanosensory responsivity in behaving mice.
Optogenetic recruitment of dorsal raphe serotonergic neurons acutely decreases mechanosensory responsivity in behaving mice.
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DOI：
10.1371/journal.pone.0105941
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Mainen ZF
中科院分区：
文献类型：
--
作者：
Dugué GP;Lörincz ML;Lottem E;Audero E;Matias S;Correia PA;Léna C;Mainen ZF
感觉反应性的抑制被认为是5-羟色胺的核心功能，但由于方法学障碍，这一假说缺乏直接支持。我们采用了一种光遗传学方法来诱导中缝背侧5-羟色胺能神经元的急性、强健和特异的放电。在体外，单个中缝背侧5-羟色胺能神经元对一连串光脉冲的反应因频率和强度以及细胞之间的不同而不同，因此光刺激方案被调整以使它们的总输出率最大化。在体内，中缝背侧5-羟色胺能神经元的光激活引起显著的光诱发场反应，对5-HT1a激动剂表现出一定的敏感性，这与中缝神经元的自身受体抑制一致。在行为正常的小鼠中，中缝背侧5-羟色胺能神经元的光刺激使动物对足底刺激的反应迅速且可逆地下降，为5-羟色胺开启感觉驱动的反应提供了新的证据。
The inhibition of sensory responsivity is considered a core serotonin function, yet this hypothesis lacks direct support due to methodological obstacles. We adapted an optogenetic approach to induce acute, robust and specific firing of dorsal raphe serotonergic neurons. In vitro, the responsiveness of individual dorsal raphe serotonergic neurons to trains of light pulses varied with frequency and intensity as well as between cells, and the photostimulation protocol was therefore adjusted to maximize their overall output rate. In vivo, the photoactivation of dorsal raphe serotonergic neurons gave rise to a prominent light-evoked field response that displayed some sensitivity to a 5-HT1A agonist, consistent with autoreceptor inhibition of raphe neurons. In behaving mice, the photostimulation of dorsal raphe serotonergic neurons produced a rapid and reversible decrease in the animals' responses to plantar stimulation, providing a new level of evidence that serotonin gates sensory-driven responses.
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