Expression of microRNAs in the stem cell niche of the adult mouse incisor.
Expression of microRNAs in the stem cell niche of the adult mouse incisor.
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DOI：
10.1371/journal.pone.0024536
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发表时间：
2011
期刊：
影响因子：
3.7
通讯作者：
Klein OD
中科院分区：
文献类型：
--
作者：
Jheon AH;Li CY;Wen T;Michon F;Klein OD
小鼠门牙是研究成体干细胞行为的一个有价值但尚未充分利用的模型器官。这颗引人注目的牙齿在动物的一生中不断生长，并容纳两个不同的上皮干细胞壁龛，分别称为唇和舌颈环（laCL和liCL）。这些干细胞产生的后代在成为产生珐琅质的成釉细胞的过程中经历一系列明确的分化事件。在分化过程中，后代从干细胞壁龛中移出，向牙齿远端迁移。虽然参与牙齿发育的分子途径已被很好地记录下来，但对mirna在这一过程中的作用知之甚少。我们使用微阵列技术比较了mirna在成年小鼠门牙的三个区域的表达：laCL、liCL和成釉细胞。我们分别从laCL/liCL和laCL/成釉细胞比较中鉴定出26个和35个差异表达的mirna。在qPCR选择的10个mirna中，所有转录本都被证实是差异表达的。原位杂交和靶标预测分析进一步支持了微阵列结果的可靠性。这些研究指出，在干细胞驱动的门牙生长过程中，mirna可能在成体干细胞的更新和分化中发挥作用。
The mouse incisor is a valuable but under-utilized model organ for studying the behavior of adult stem cells. This remarkable tooth grows continuously throughout the animal's lifetime and houses two distinct epithelial stem cell niches called the labial and lingual cervical loop (laCL and liCL, respectively). These stem cells produce progeny that undergo a series of well-defined differentiation events en route to becoming enamel-producing ameloblasts. During this differentiation process, the progeny move out of the stem cell niche and migrate toward the distal tip of the tooth. Although the molecular pathways involved in tooth development are well documented, little is known about the roles of miRNAs in this process. We used microarray technology to compare the expression of miRNAs in three regions of the adult mouse incisor: the laCL, liCL, and ameloblasts. We identified 26 and 35 differentially expressed miRNAs from laCL/liCL and laCL/ameloblast comparisons, respectively. Out of 10 miRNAs selected for validation by qPCR, all transcripts were confirmed to be differentially expressed. In situ hybridization and target prediction analyses further supported the reliability of our microarray results. These studies point to miRNAs that likely play a role in the renewal and differentiation of adult stem cells during stem cell-fueled incisor growth.
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