Novel DNA-binding element within the C-terminal extension of the nuclear receptor DNA-binding domain.
Novel DNA-binding element within the C-terminal extension of the nuclear receptor DNA-binding domain.
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DOI：
10.1093/nar/gkm162
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发表时间：
2007
影响因子：
14.9
通讯作者：
Ozyhar A
中科院分区：
文献类型：
--
作者：
Jakób M;Kołodziejczyk R;Orłowski M;Krzywda S;Kowalska A;Dutko-Gwóźdź J;Gwóźdź T;Kochman M;Jaskólski M;Ozyhar A
蜕皮激素受体(ECR)和超阿司匹林(USP)的异源二聚体是核受体超家族的成员，被认为是蜕皮激素启动昆虫蜕皮和变态的功能性受体。在这里，我们报告了由DNA结合结构域(DBD)、ECR和USP形成的复合体的1.95 ä结构，该复合体与天然的伪脂水反应元件结合。将该结构与以前使用理想化反应元件获得的结构进行比较，显示了先前报道具有非凡灵活性的EcRDBD如何适应DNA诱导的结构变化。ECRDBD的部分C末端延伸折叠成α-螺旋，其在小沟中的位置与先前观察到的核受体的任何位置都不匹配。突变分析表明，α-螺旋是ECR-box的组成部分，ECR-box是一种新的DNA结合所必需的元件，位于核受体CTE内。这一要素似乎是所有已知ECR的一个普遍特征。
The heterodimer of the ecdysone receptor (EcR) and ultraspiracle (Usp), members of the nuclear receptors superfamily, is considered as the functional receptor for ecdysteroids initiating molting and metamorphosis in insects. Here we report the 1.95 Å structure of the complex formed by the DNA-binding domains (DBDs) the EcR and the Usp, bound to the natural pseudopalindromic response element. Comparison of the structure with that obtained previously, using an idealized response element, shows how the EcRDBD, which has been previously reported to possess extraordinary flexibility, accommodates DNA-induced structural changes. Part of the C-terminal extension (CTE) of the EcRDBD folds into an α-helix whose location in the minor groove does not match any of the locations previously observed for nuclear receptors. Mutational analyses suggest that the α-helix is a component of EcR-box, a novel element indispensable for DNA-binding and located within the nuclear receptor CTE. This element seems to be a general feature of all known EcRs.
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DOI：
10.1107/s0907444998012517
发表时间：
1999-02-01
影响因子：
2.2
作者：
Kissinger, CR;Gehlhaar, DK;Fogel, DB
通讯作者：
Fogel, DB
影响因子：
2.6
作者：
BARIK, S
通讯作者：
BARIK, S
影响因子：
64.5
作者：
KOELLE, MR;TALBOT, WS;HOGNESS, DS
通讯作者：
HOGNESS, DS
DOI：
10.1046/j.1432-1327.2001.02287.x
发表时间：
2001-07-01
期刊：
EUROPEAN JOURNAL OF BIOCHEMISTRY
影响因子：
--
作者：
Grad, I;Niedziela-Majka, A;Ozyhar, A
通讯作者：
Ozyhar, A
影响因子：
56.9
作者：
EVANS, RM
通讯作者：
EVANS, RM