Hydrodynamic optical alignment for microflow cytometry.
Hydrodynamic optical alignment for microflow cytometry.
复制标题
DOI：
10.1039/c0lc00500b
复制
发表时间：
2011-03-21
期刊：
影响因子：
6.1
通讯作者：
Batt CA
中科院分区：
文献类型：
--
作者：
Kennedy MJ;Stelick SJ;Sayam LG;Yen A;Erickson D;Batt CA
已经开发了一种微制造的流式细胞仪，其能够检测水性悬浮液中几乎所有的微粒。目前的设计允许基于光纤的检测系统和粒子流之间经由流体动力学聚焦的集成耦合。通过调节聚焦歧管的辅助输入端处的相对流速，颗粒流可以相对于照射激光器被引导出平面，并且类似地，颗粒流可以被挤压或膨胀。微加工器件由聚二甲基硅氧烷制成，微流控截面尺寸为125 μm × 125 μm。使用本装置和方法，以91 ± 4%的绝对计数效率对水溶液中的荧光微粒进行计数。远红色荧光微粒的荧光脉冲高度的变异系数为15%。该装置表现出对荧光强度校准微粒的线性响应，如通过与商业细胞仪仪器比较所示。
A microfabricated flow cytometer has been developed that is capable of detecting nearly all of the microparticles in an aqueous suspension. Current design allows for integrated coupling between an optical fiber-based detection system and the particle stream via hydrodynamic focusing. By adjusting the relative flow-rates at the auxiliary inputs of the focusing manifold, the particle stream can be steered out-of-plane relative to the illuminating laser, and similarly the particle stream can be squeezed or expanded. The microfabricated device was constructed in polydimethylsiloxane with cross-sectional microfluidic dimensions of 125 μm × 125 μm. Using the present device and method, fluorescent microparticles in aqueous solution were counted at an absolute counting efficiency of 91 ± 4%. The coefficient of variation of the fluorescence pulse-heights for far-red fluorescent microparticles was 15%. The device exhibited a linear response to fluorescence intensity calibration microparticles as shown by comparison with a commercial cytometer instrument.
登录
查看更多内容
影响因子：
2.8
作者：
Rosenauer, Michael;Buchegger, Wolfgang;Vellekoop, Michael
通讯作者：
Vellekoop, Michael
DOI：
10.1073/pnas.0404423101
发表时间：
2004-08-24
影响因子：
11.1
作者：
Wolfe, DB;Conroy, RS;Whitesides, GM
通讯作者：
Whitesides, GM
使用凹槽生成的护套流量的多波长微流动仪。
影响因子：
6.1
作者：
Golden JP;Kim JS;Erickson JS;Hilliard LR;Howell PB;Anderson GP;Nasir M;Ligler FS
通讯作者：
Ligler FS
影响因子：
8.4
作者：
Tung, YC;Zhang, M;Skerlos, SJ
通讯作者：
Skerlos, SJ
影响因子：
7.4
作者：
Kim, Jason S.;Anderson, George P.;Erickson, Jeffrey S.;Golden, Joel P.;Nasir, Mansoor;Ligler, Frances S.
通讯作者：
Ligler, Frances S.