Multi-wavelength microflow cytometer using groove-generated sheath flow.
Multi-wavelength microflow cytometer using groove-generated sheath flow.
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使用凹槽生成的护套流量的多波长微流动仪。
DOI：
10.1039/b822442k
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发表时间：
2009-07-07
期刊：
影响因子：
6.1
通讯作者：
Ligler FS
中科院分区：
文献类型：
--
作者：
Golden JP;Kim JS;Erickson JS;Hilliard LR;Howell PB;Anderson GP;Nasir M;Ligler FS
开发了一种微流式细胞仪，其在所有侧面上包封样品（核心）流体，并在四个不同波长下询问样品流中的每个颗粒。通过首先经由流体聚焦将芯流体与鞘流体横向地混合来实现鞘化。在通道的顶部和底部中制造的人字形凹槽特征将鞘液从通道的侧面引导到顶部和底部，完全包围样品流。插入到引导通道中的光纤提供来自二极管激光器的532和635 nm的激发光，并收集发射波长。两个发射收集光纤通过多模光纤分路器和滤光器连接到PMT，用于在635 nm（散射）、665 nm和700 nm（微球识别）以及565 nm（藻红蛋白示踪剂）处检测。细胞计数器能够区分具有不同量的荧光团的微球，所述荧光团用于编码和检测微球表面上藻红蛋白抗体复合物的存在。大肠杆菌的测定与商业Luminex流式细胞仪进行了比较。
A microflow cytometer was developed that ensheathed the sample (core) fluid on all sides and interrogated each particle in the sample stream at four different wavelengths. Sheathing was achieved by first sandwiching the core fluid with the sheath fluid laterally via fluid focusing. Chevron-shaped groove features fabricated in the top and bottom of the channel directed sheath fluid from the sides to the top and bottom of the channel, completely surrounding the sample stream. Optical fibers inserted into guide channels provided excitation light from diode lasers at 532 and 635 nm and collected the emission wavelengths. Two emission collection fibers were connected to PMTs through a multimode fiber splitter and optical filters for detection at 635 nm (scatter), 665 nm and 700 nm (microsphere identification) and 565 nm (phycoerythrin tracer). The cytometer was capable of discriminating microspheres with different amounts of the fluorophores used for coding and detecting the presence of a phycoerythrin antibody complex on the surface of the microspheres. Assays for Escherichia coli were compared with a commercial Luminex flow cytometer.
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2.7
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