Metabolic and structural integrity of magnetic nanoparticle-loaded primary endothelial cells for targeted cell therapy.
Metabolic and structural integrity of magnetic nanoparticle-loaded primary endothelial cells for targeted cell therapy.
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DOI：
10.2217/nnm.15.14
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发表时间：
2015-05
期刊：
影响因子：
--
通讯作者：
Polyak B
中科院分区：
文献类型：
--
作者：
Orynbayeva Z;Sensenig R;Polyak B
为了成功地将磁性介导的细胞靶向从实验室转移到床边，需要系统地评估磁性纳米颗粒（MNP）与“治疗性”细胞相互作用的潜在不良影响。在这里，我们详细研究了内化的聚合物MNP对原代大鼠内皮细胞的结构完整性，代谢完整性和增殖潜力的影响。通过荧光共聚焦显微镜、流式细胞仪和高分辨率呼吸仪研究细胞骨架和细胞器的完整性。MNP负载的原代内皮细胞保持完整的细胞骨架和细胞器，维持正常的增殖速率、钙信号和线粒体能量代谢。本研究提供了支持性证据，即靶向所需剂量的MNP不会对原代内皮细胞-潜在细胞治疗载体的结构完整性和功能性产生显著不良影响。
To successfully translate magnetically mediated cell targeting from bench to bedside, there is a need to systematically assess the potential adverse effects of magnetic nanoparticles (MNPs) interacting with ‘therapeutic’ cells. Here, we examined in detail the effects of internalized polymeric MNPs on primary rat endothelial cells’ structural intactness, metabolic integrity and proliferation potential. The intactness of cytoskeleton and organelles was studied by fluorescent confocal microscopy, flow cytometry and high-resolution respirometry. MNP-loaded primary endothelial cells preserve intact cytoskeleton and organelles, maintain normal rate of proliferation, calcium signaling and mitochondria energy metabolism. This study provides supportive evidence that MNPs at doses necessary for targeting did not induce significant adverse effects on structural integrity and functionality of primary endothelial cells – potential cell therapy vectors.
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影响因子：
9.2
作者：
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通讯作者：
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