Force dependent internalization of magnetic nanoparticles results in highly loaded endothelial cells for use as potential therapy delivery vectors.
Force dependent internalization of magnetic nanoparticles results in highly loaded endothelial cells for use as potential therapy delivery vectors.
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DOI：
10.1007/s11095-011-0663-7
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发表时间：
2012-05
影响因子：
3.7
通讯作者：
Polyak, Boris
中科院分区：
文献类型：
--
作者：
MacDonald, Cristin;Barbee, Kenneth;Polyak, Boris
研究MNP负载到内皮细胞中的动力学、机制和程度以及这种负载对细胞功能的影响。MNP吸收进行了检查，在现场开/关条件下，利用不同的磁铁矿浓度MNP。使用代谢呼吸、细胞增殖和迁移测定评估MNP负载的细胞活力和功能完整性。与非磁性条件相比，在磁场的影响下，内皮细胞中的MNP摄取显著增加。更大的磁铁矿密度的MNP导致更高的MNP内化下的磁场的应用程序中的细胞，而不损害细胞呼吸活性。二维迁移试验表明，在没有字段更高的磁铁矿负载导致更大的细胞迁移率。在磁场下的三维迁移试验中，MNP负载细胞的迁移率是未负载细胞的两倍以上，并且与血清梯度刺激的迁移相当。我们的研究结果表明，内皮细胞摄取MNP是一个力依赖性的过程。体外试验确定细胞健康不会受到高MNP负载的不利影响，从而使这些高度磁响应的细胞成为潜在有益的治疗（基因、药物或细胞）递送系统。
To investigate the kinetics, mechanism and extent of MNP loading into endothelial cells and the effect of this loading on cell function. MNP uptake was examined under field on/off conditions, utilizing varying magnetite concentration MNPs. MNP-loaded cell viability and functional integrity was assessed using metabolic respiration, cell proliferation and migration assays. MNP uptake in endothelial cells significantly increased under the influence of a magnetic field versus non-magnetic conditions. Larger magnetite density of the MNPs led to a higher MNP internalization by cells under application of a magnetic field without compromising cellular respiration activity. Two-dimensional migration assays at no field showed that higher magnetite loading resulted in greater cell migration rates. In a three-dimensional migration assay under magnetic field, the migration rate of MNP-loaded cells was more than twice that of unloaded cells and was comparable to migration stimulated by a serum gradient. Our results suggest that endothelial cell uptake of MNPs is a force dependent process. The in vitro assays determined that cell health is not adversely affected by high MNP loadings, allowing these highly magnetically responsive cells to be potentially beneficial therapy (gene, drug or cell) delivery systems.
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影响因子：
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