Low UVB Fluences Augment Microvesicle Particle Generation in Keratinocytes.
Low UVB Fluences Augment Microvesicle Particle Generation in Keratinocytes.
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低UVB量增加角质形成细胞中的微泡颗粒的产生。
DOI：
10.1111/php.13495
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发表时间：
2022-01
影响因子：
3.3
通讯作者：
Travers JB
中科院分区：
文献类型：
--
作者：
Thapa P;Bhadri S;Borchers C;Liu L;Chen Y;Rapp CM;Travers JB
微泡颗粒（MVP）是一种具有生物活性的亚细胞颗粒，最近被认为与角质细胞对许多环境应激源的反应有关，包括紫外线B辐射（UVB）。先前的研究表明，UVB在一个涉及血小板活化因子受体（PAFR）和酸性鞘磷脂酶（aSMase）的过程中产生高水平的MVP。然而，产生MVP所需的UVB的影响通常高于常见的影响。通过体外人上皮细胞系、体外人皮肤外植体和小鼠体内研究，本研究表明，用PAFR激动剂/佛波酯预处理上皮细胞/皮肤可与低UVB协同产生高水平的MVP。这些研究表明MVP可能在与中波紫外线有关的组合病理过程中发挥作用。PAFR激动剂+ UVB联合产生高水平MVP的假设模型。在该模型中，单独使用UVB或PAFR激动剂（或phorbol酯）可导致低水平的MVP释放。然而，这些不同刺激的结合导致高水平的MVP形成和释放。
Microvesicle particles (MVP) are bioactive subcellular particles which have been recently implicated in the keratinocyte response to many environmental stressors including ultraviolet B radiation (UVB). Previous studies have demonstrated that UVB generates high levels of MVP in a process involving the Platelet-activating factor receptor (PAFR) and the enzyme acid sphingomyelinase (aSMase). Yet the fluences of UVB needed to generate MVP are usually above those commonly encountered. Using models including human epithelial cell lines in vitro, human skin explants ex vivo and murine studies in vivo, the present studies indicate that pretreatment of epithelial cells/skin with PAFR agonist/phorbol ester can synergize with low fluences of UVB to generate high levels of MVP. These studies indicate the possibility that MVP could play a role in combinatorial pathologic processes involving UVB. Hypothetical model by which combining PAFR agonists + UVB generates high levels of MVP. In this model, UVB or PAFR agonists (or phorbol esters) alone result in low levels of MVP release. However, the combination of these disparate stimuli results in high levels of MVP formation and release.
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影响因子：
3.3
作者：
Bihl JC;Rapp CM;Chen Y;Travers JB
通讯作者：
Travers JB
影响因子：
11.4
作者：
Bianco, Fabio;Perrotta, Cristiana;Novellino, Luisa;Francolini, Maura;Riganti, Loredana;Menna, Elisabetta;Saglietti, Laura;Schuchman, Edward H.;Furlan, Roberto;Clementi, Emilio;Matteoli, Michela;Verderio, Claudia
通讯作者：
Verderio, Claudia
影响因子：
4.4
作者：
Liu, Langni;Fahy, Katherine E.;Travers, Jeffrey B.
通讯作者：
Travers, Jeffrey B.
影响因子：
4.8
作者：
Marathe, GK;Johnson, C;Travers, JB
通讯作者：
Travers, JB
影响因子：
3.3
作者：
Fahy K;Liu L;Rapp CM;Borchers C;Bihl JC;Chen Y;Simman R;Travers JB
通讯作者：
Travers JB