Dynamic sodium imaging at ultra-high field reveals progression in a preclinical migraine model.
Dynamic sodium imaging at ultra-high field reveals progression in a preclinical migraine model.
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DOI：
10.1097/j.pain.0000000000001307
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发表时间：
2018-10
期刊：
影响因子：
7.4
通讯作者：
Grant SC
中科院分区：
文献类型：
--
作者：
Abad N;Rosenberg JT;Hike DC;Harrington MG;Grant SC
在偏头痛的发生和发展过程中，细胞外钠的增加导致持续的神经元兴奋性的假设下，这项研究评估了临床前偏头痛的啮齿动物类似物在体内的动态23Na流量。在中枢敏化开始后，21.1T的超高场~(23)Na磁共振成像显示了以良好的空间和时间分辨率量化钠浓度的潜力。采用原位注射硝酸甘油(NTG)10 mg/kg的雄性SD大鼠，以空白对照组和生理盐水对照组为对照，对植入IP线的SD大鼠进行了MRI检查。使用基于多层FID的化学位移成像序列，以1.1×1.1×3 mm的分辨率采集23Na图像，采集时间为9min。在基线扫描的1小时内和注射后3小时的纵向扫描中，总共获得了27次重复扫描。注射NTG后即刻，脑干、脑外脑脊液和枕大池的~(23)Na MRI信号明显增强，与对照组比较差异有统计学意义(P<0.05)。小脑和第三脑室也有持续升高的趋势，前者在注射NTG后2小时以上开始显著升高。这些数据提供了钠浓度早期变化的证据，尤其是在后颅窝脑脊液和脑干区域。进一步研究钠浓度的波动及其与治疗的调节有助于了解偏头痛的动态特征、偏头痛发生者的位置，并指导制定纠正钠稳态紊乱的治疗措施。
Under the hypothesis that increased extracellular sodium induces sustained neuronal excitability with the onset and progression of migraine, this study evaluates dynamic in vivo 23Na fluxes in the brain of a preclinical rodent analogue of migraine. Ultra-high field 23Na MRI at 21.1 T has demonstrated potential to quantify sodium concentrations with good spatial and temporal resolution following the onset of central sensitization. Sprague-Dawley male rats with implanted intraperitoneal (IP) lines were studied by MRI before and after an in situ injection of 10 mg/kg of nitroglycerin (NTG) versus vehicle and saline controls. Slice-selective 23Na images were acquired using a multi-slice FID-based Chemical Shift Imaging sequence with resolution of 1.1 × 1.1 × 3 mm for a 9-min acquisition. A total of twenty-seven repeated scans were acquired over 1 h of baseline scanning and longitudinally up to 3 h post injection. Increases of 23Na MRI signal in the brainstem, extra-cerebral cerebrospinal fluid (CSF) and cisterna magna were evident almost immediately after NTG injection, gaining significance from controls in 36 min. The cerebellum and third ventricle also showed sustained trends of increased 23Na, with the former gaining significance at over 2 h post-NTG injection. The data provide evidence of an early change in sodium concentration, markedly in posterior fossa CSF and brainstem regions. Further study of fluctuations of sodium concentration and their modulation with treatments could help understand the dynamic features of migraine, location of a putative migraine generator, and guide development of therapeutic measures to correct the disturbance of sodium homeostasis.
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影响因子：
4.9
作者：
Greco, Rosaria;Meazza, Cristina;Tassorelli, Cristina
通讯作者：
Tassorelli, Cristina
影响因子：
14.5
作者：
Afridi, SK;Matharu, MS;Goadsby, PJ
通讯作者：
Goadsby, PJ
影响因子：
2.5
作者：
de Tommaso, M;Libro, G;Cerbo, R
通讯作者：
Cerbo, R
影响因子：
8.3
作者：
ASTRUP, J;SORENSEN, PM;SORENSEN, HR
通讯作者：
SORENSEN, HR
硝酸甘油给药后特定大脑区域中ADMA-DDAH-NOS轴的评估：在偏头痛动物模型中进行研究。
DOI：
10.1186/s10194-015-0560-2
发表时间：
2015
期刊：
The journal of headache and pain
影响因子：
--
作者：
Greco R;Ferrigno A;Demartini C;Zanaboni A;Mangione AS;Blandini F;Nappi G;Vairetti M;Tassorelli C
通讯作者：
Tassorelli C