T Cells Development Is Different between Thymus from Normal and Intrauterine Growth Restricted Pig Fetus at Different Gestational Stage.
T Cells Development Is Different between Thymus from Normal and Intrauterine Growth Restricted Pig Fetus at Different Gestational Stage.
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不同妊娠期正常猪胎儿与宫内生长受限猪胎儿胸腺的 T 细胞发育存在差异
DOI：
10.5713/ajas.2012.12132
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发表时间：
2013-03
期刊：
影响因子：
--
通讯作者：
Lai C
中科院分区：
文献类型：
--
作者：
Lin Y;Wang J;Wang X;Wu W;Lai C
本试验旨在研究不同妊娠期IUGR仔猪T细胞的发育情况，并初步探讨T细胞发育与Notch信号通路的关系。共18头杂交（长白猪×大白色）经产母猪在相似的体重和发情期交配，并在妊娠第60、90和110天处以安乐死，每个时间点重复6次。从每窝中挑选一只IUGR和一只正常胎儿。检测胸腺T细胞亚群、Delta-like 1、Delta-like 4、Jagged 1和Notch 2基因mRNA表达。与正常仔猪相比，IUGR胎儿在第90天的CD 3 + CD 4 − CD 8+细胞降低了0.13%（p<0.05）。IUGR胎儿CD 8 + T细胞在孕110天较正常胎儿低0.19%（p<0.05）。IUGR猪60 d时，CD 8 + T细胞占总T细胞的比例降低了3.14%（p<0.05）。与正常猪相比，Notch 2和Delta-like 4 mRNA在第110天的丰度分别增加20.93%和减少0.77%（p<0.05），Delta-like 1 mRNA在第90天的丰度增加0.19%（p<0.05）。这些结果表明，正常胎儿在妊娠早期具有更大比例的T细胞亚群，并且Notch信号通路可能在一定程度上部分负责这些差异。
This experiment was conducted to evaluate the development of T cells in intrauterine growth retarded (IUGR) piglets at different gestational stages, and tentatively explore the relationship between T cells development and the Notch signaling pathway. A total of 18 crossbred (Landrace×Large white) primiparous sows were mated at similar weights and estruses and euthanized at d 60, 90 and 110 of gestation with six replicates for each time point. One IUGR and one normal fetus were picked from each litter. The T-cell subsets, mRNA expression of Delta-like1, Delta-like4, Jagged1, and Notch2 genes in the thymus were investigated. Compared to normal piglets, CD3+CD4−CD8+ cells in IUGR fetuses at d 90 was 0.13% lower (p<0.05). At d 110 of gestation CD8+ T cells in IUGR fetuses was 0.19% lower (p<0.05). The percentage of CD8+ T cells was 3.14% lower (p<0.05) of the total T cells in IUGR pigs at d 60. The abundance of Notch2 and Delta-like4 mRNA at d 110 was 20.93% higher and 0.77% (p<0.05) lower, and Delta-like1 mRNA at d 90 was 0.19% (p<0.05) higher compared to normal pigs. These results suggested that normal fetuses had a greater proportion of T-cell subsets at earlier gestation periods, and the Notch signaling pathway was likely partially responsible for these differences to some degree.
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DOI：
10.1001/archpedi.1975.02120410038013
发表时间：
1975-01-01
影响因子：
--
作者：
CHANDRA, RK
通讯作者：
CHANDRA, RK
影响因子：
4.5
作者：
Dacasto, M;Cornaglia, E;Bollo, E
通讯作者：
Bollo, E
影响因子：
5.1
作者：
FERGUSON, AC;LAWLOR, GJ;STIEHM, ER
通讯作者：
STIEHM, ER
影响因子：
2.6
作者：
Lefort, Nathalie;Benne, Clarisse;Levy, Yves
通讯作者：
Levy, Yves
影响因子：
4.5
作者：
Neumann, CG;Stiehm, ER;Karuga-Koinange, W
通讯作者：
Karuga-Koinange, W