Transcriptomic and metabolomic profiles of Chinese citrus fly, Bactrocera minax (Diptera: Tephritidae), along with pupal development provide insight into diapause program.
Transcriptomic and metabolomic profiles of Chinese citrus fly, Bactrocera minax (Diptera: Tephritidae), along with pupal development provide insight into diapause program.
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中国柑橘蝇 Bactrocera minax（双翅目：实蝇科）的转录组学和代谢组学特征以及蛹的发育为滞育计划提供了深入的了解
DOI：
10.1371/journal.pone.0181033
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发表时间：
2017
期刊：
影响因子：
3.7
通讯作者：
Liu YH
中科院分区：
文献类型：
--
作者：
Wang J;Fan H;Xiong KC;Liu YH
中国柑橘蝇 Bactrocera minax (Enderlein) 是亚洲的一种毁灭性柑橘害虫。这种单性昆虫在每一代都会进入强制性的蛹滞育，但人们对滞育的过程和分子机制知之甚少。在这项研究中，滞育过程是通过测量整个蛹阶段的呼吸频率来确定的。此外，分别通过新一代测序技术和1H核磁共振波谱（NMR）评估了蛹在五个发育阶段（滞育前、早期、中期、晚期和滞育后）转录组和代谢组谱的变化。在十对比较中，共有 4,808 个基因发生显着改变，代表新陈代谢和信号转导以及内分泌系统和消化系统的重大变化。通过 qRT-PCR 分析验证基因表达谱。此外，还通过 1 H NMR 鉴定并定量了 48 种代谢物。其中九种对代谢组谱的变化有显着影响，尤其是脯氨酸和海藻糖。此外，在滞育维持期间（滞育早期、中期和晚期）收集的样本仅表现出彼此之间的边缘转录组和代谢组变化。这些发现极大地提高了我们对B. minax滞育的认识，为进一步的相关研究奠定了基础。
The Chinese citrus fly, Bactrocera minax (Enderlein), is a devastating citrus pest in Asia. This univoltine insect enters obligatory pupal diapause in each generation, while little is known about the course and the molecular mechanisms of diapause. In this study, the course of diapause was determined by measuring the respiratory rate throughout the pupal stage. In addition, the variation of transcriptomic and metabolomic profiles of pupae at five developmental stages (pre-, early-, middle-, late-, and post-diapause) were evaluated by next-generation sequencing technology and 1H nuclear magnetic resonance spectroscopy (NMR), respectively. A total of 4,808 genes were significantly altered in ten pairwise comparisons, representing major shifts in metabolism and signal transduction as well as endocrine system and digestive system. Gene expression profiles were validated by qRT-PCR analysis. In addition, 48 metabolites were identified and quantified by 1H NMR. Nine of which significantly contributed to the variation in the metabolomic profiles, especially proline and trehalose. Moreover, the samples collected within diapause maintenance (early-, middle-, and late-diapause) only exhibited marginal transcriptomic and metabolomic variation with each other. These findings greatly improve our understanding of B. minax diapause and lay the foundation for further pertinent studies.
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