Two-dimensional paper network format that enables simple multistep assays for use in low-resource settings in the context of malaria antigen detection.
Two-dimensional paper network format that enables simple multistep assays for use in low-resource settings in the context of malaria antigen detection.
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DOI：
10.1021/ac300689s
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发表时间：
2012-05-15
影响因子：
7.4
通讯作者：
Yager, Paul
中科院分区：
文献类型：
--
作者：
Fu, Elain;Liang, Tinny;Spicar-Mihalic, Paolo;Houghtaling, Jared;Ramachandran, Sujatha;Yager, Paul
横向流动试验已成为低资源环境下的标准生物测定格式，因为它快速、易于使用、成本低、使用干燥形式储存的试剂，并且不需要设备。然而，横向流动测试通常局限于单一的化学输送步骤，而不具备高性能实验室分析的多步骤处理特征。为了解决这一限制，我们正在开发一个纸质网络平台，将传统的横向流动测试扩展到二维；这允许合并多步骤化学处理，同时仍然保留传统横向流动测试的优点。在这里，我们展示了一种易于使用的，信号放大的三明治格式疟疾蛋白PfHRP2免疫分析。卡片中含有干燥形式的试剂，因此用户只需要添加样品和水。该设备内的多个流程在单个用户折叠卡关闭的步骤中被激活；纸网络的配置自动递送适当体积的i)样品和偶联到金颗粒标签的抗体，ii)冲洗缓冲液，iii)信号放大试剂到捕获区域。这些结果突出了论文网络平台在发达国家和发展中国家资源匮乏环境中提高获得高质量诊断能力的潜力。
The lateral flow test has become the standard bioassay format in low-resource settings because it is rapid, easy to use, low in cost, uses reagents stored in dry form, and is equipment-free. However, lateral flow tests are often limited to a single chemical delivery step and not capable of the multi-step processing characteristic of high performance laboratory-based assays. To address this limitation, we are developing a paper network platform that extends the conventional lateral flow test to two dimensions; this allows incorporation of multi-step chemical processing, while still retaining the advantages of conventional lateral flow tests. Here we demonstrate this format for an easy-to-use, signal-amplified sandwich format immunoassay for the malaria protein PfHRP2. The card contains reagents stored in dry form such that the user need only add sample and water. The multiple flows in the device are activated in a single user step of folding the card closed; the configuration of the paper network automatically delivers the appropriate volumes of i) sample plus antibody conjugated to a gold particle label, ii) a rinse buffer, and iii) a signal amplification reagent to the capture region. These results highlight the potential of the paper network platform to enhance access to high-quality diagnostic capabilities in low-resource settings in the developed and developing worlds.
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DOI：
10.1007/978-1-59745-240-3_1
发表时间：
2008-10-31
期刊：
Lateral Flow Immunoassay
影响因子：
--
作者：
O’Farrell B
通讯作者：
O’Farrell B
影响因子：
2.8
作者：
Yeh, Chia-Hsien;Hung, Ching-Yuan;Lin, Yu-Cheng
通讯作者：
Lin, Yu-Cheng
影响因子：
6.2
作者：
Dungchai, Wijitar;Chailapakul, Orawon;Henry, Charles S.
通讯作者：
Henry, Charles S.
影响因子：
6.1
作者：
Zhang, C;Zhang, Y;Wang, S
通讯作者：
Wang, S
影响因子：
7.4
作者：
Cho, JH;Han, SM;Paek, SH
通讯作者：
Paek, SH