De novo transcriptomic analysis of an oleaginous microalga: pathway description and gene discovery for production of next-generation biofuels.
De novo transcriptomic analysis of an oleaginous microalga: pathway description and gene discovery for production of next-generation biofuels.
复制标题
DOI：
10.1371/journal.pone.0035142
复制
发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Zhang C
中科院分区：
文献类型：
--
作者：
Wan L;Han J;Sang M;Li A;Wu H;Yin S;Zhang C
Eustigmatos cf. polypem是一种黄绿色的单细胞土壤微生物，属于具有高生物量和大量生产用于生物燃料的三酰基甘油（TAG）的真核植物，因此被称为产油微生物。然而，微藻基因组序列的缺乏限制了基于基因的生物燃料原料优化研究的发展。在这里，我们描述了测序和从头转录组组装的非模式微藻物种，E。参见polyphem，并确定与生物燃料生产相关的重要途径和基因。我们进行了E.参见使用Illumina配对末端测序技术对polypem转录组进行测序。在单次运行中，我们产生了对应于2.33Gb总核苷酸的29，199，432个测序读段。将这些读段组装成75，632个单基因，平均大小为503 bp，N50为663 bp，范围为100 bp至> 3，000 bp。对组装的单基因进行BLAST相似性搜索，并用基因本体论（GO）和京都基因和基因组百科全书（KEGG）同源性标识符进行注释。这些分析确定了大肠杆菌中碳水化合物、脂肪酸、TAG和类胡萝卜素的生物合成和催化途径。参见聚血红素我们的数据提供了参与碳水化合物，脂肪酸，TAG和类胡萝卜素的生物合成和催化的代谢途径的构建。参见本发明的目的是提供一种新的多聚体生物燃料，并为指导代谢工程努力所需的分子遗传学和功能基因组学提供基础，所述代谢工程努力寻求提高基于微藻的生物燃料原料的数量和特性。
Eustigmatos cf. polyphem is a yellow-green unicellular soil microalga belonging to the eustimatophyte with high biomass and considerable production of triacylglycerols (TAGs) for biofuels, which is thus referred to as an oleaginous microalga. The paucity of microalgae genome sequences, however, limits development of gene-based biofuel feedstock optimization studies. Here we describe the sequencing and de novo transcriptome assembly for a non-model microalgae species, E. cf. polyphem, and identify pathways and genes of importance related to biofuel production. We performed the de novo assembly of E. cf. polyphem transcriptome using Illumina paired-end sequencing technology. In a single run, we produced 29,199,432 sequencing reads corresponding to 2.33 Gb total nucleotides. These reads were assembled into 75,632 unigenes with a mean size of 503 bp and an N50 of 663 bp, ranging from 100 bp to >3,000 bp. Assembled unigenes were subjected to BLAST similarity searches and annotated with Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) orthology identifiers. These analyses identified the majority of carbohydrate, fatty acids, TAG and carotenoids biosynthesis and catabolism pathways in E. cf. polyphem. Our data provides the construction of metabolic pathways involved in the biosynthesis and catabolism of carbohydrate, fatty acids, TAG and carotenoids in E. cf. polyphem and provides a foundation for the molecular genetics and functional genomics required to direct metabolic engineering efforts that seek to enhance the quantity and character of microalgae-based biofuel feedstock.
登录
查看更多内容
影响因子：
56.9
作者：
Hites, RA;Foran, JA;Schwager, SJ
通讯作者：
Schwager, SJ
影响因子：
2.8
作者：
Dinh, Linh T. T.;Guo, Yuyan;Mannan, M. Sam
通讯作者：
Mannan, M. Sam
DOI：
10.1073/pnas.120067297
发表时间：
2000-06-06
影响因子：
11.1
作者：
Dahlqvist, A;St책hl, U;Stymne, H
通讯作者：
Stymne, H
影响因子：
5.4
作者：
Guedes AC;Amaro HM;Malcata FX
通讯作者：
Malcata FX
影响因子：
5.8
作者：
Conesa, A;Götz, S;Robles, M
通讯作者：
Robles, M