Lin28a up-regulation is associated with the formation of restenosis via promoting proliferation and migration of vascular smooth muscle cells.
Lin28a up-regulation is associated with the formation of restenosis via promoting proliferation and migration of vascular smooth muscle cells.
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Lin28a 上调通过促进血管平滑肌细胞增殖和迁移与再狭窄的形成相关
DOI：
10.1111/jcmm.15506
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发表时间：
2020-09
影响因子：
5.3
通讯作者：
Dong J
中科院分区：
文献类型：
--
作者：
Zou Z;Zhou X;Zhang R;Zhang Q;Jiang S;Xu C;Zhang R;Xie T;Zhu H;Gong P;Zhang D;Ma H;Liao L;Dong J
为了探讨Lin28a在经皮腔内血管成形术后再狭窄发展中的潜在作用，我们采用双球囊损伤手术和单球囊损伤手术分别建立再狭窄和动脉粥样硬化模型，以便更好地区分再狭窄和动脉粥样硬化病变。免疫组化分析显示，Lin28a在髂动脉再狭窄斑块中的表达明显高于动脉粥样硬化斑块。免疫荧光研究显示，Lin28a与α‐平滑肌肌动蛋白共定位在再狭窄斑块中，而不是在动脉粥样硬化斑块中，这表明Lin28a可能与血管平滑肌细胞（VSMCs）在再狭窄中的独特行为有关。为了进一步证实上述假设，在培养的VSMCs中，转染Lenti‐Lin28a可上调Lin28a的表达，转染Lenti‐Lin28a‐shRNA可抑制其表达，然后分别用EdU和Transwell法检测VSMCs的增殖和迁移能力。结果表明，随着Lin28a表达的上调，VSMCs的增殖和迁移能力明显增强，而抑制Lin28a表达后，VSMCs的上述行为明显受到抑制。综上所述，Lin28a的上调对VSMCs的增殖和迁移具有调节作用，可能在再狭窄的病理形成中起关键作用。
To explore the potential role of Lin28a in the development of restenosis after percutaneous transluminal angioplasty, double‐balloon injury surgery and mono‐balloon injury surgery were used to establish restenosis and atherosclerosis models, respectively, so as to better distinguish restenosis from atherosclerotic lesions. Immunohistochemical analysis revealed that significantly higher expression of Lin28a was observed in the iliac arteries of restenosis plaques than that of atherosclerosis plaques. Immunofluorescence studies showed the colocalization of Lin28a with α‐smooth muscle actin in restenosis plaques, rather than in atherosclerosis plaques, which suggested that Lin28a might be related to the unique behaviour of vascular smooth muscle cells (VSMCs) in restenosis. To further confirm above hypothesis, Lin28a expression was up‐regulated by transfection of Lenti‐Lin28a and inhibited by Lenti‐Lin28a‐shRNA transfection in cultured VSMCs, and then the proliferation and migration capability of VSMCs were detected by EdU and Transwell assays, respectively. Results showed that the proliferation and migration of VSMCs were significantly increased in accordance with the up‐regulation of Lin28a expression, while above behaviours of VSMCs were significantly suppressed after inhibiting the expression of Lin28a. In conclusion, the up‐regulation of Lin28a exerts its modulatory effect on VSMCs’ proliferation and migration, which may play a critical role in contributing to pathological formation of restenosis.
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影响因子：
5.8
作者：
Wang D;Zhu Y;Wang Y;Li Z;Yuan C;Zhang W;Yuan H;Ye J;Yang J;Jiang H;Cheng J
通讯作者：
Cheng J
影响因子：
4.6
作者：
Tsialikas, Jennifer;Romer-Seibert, Jennifer
通讯作者：
Romer-Seibert, Jennifer
影响因子：
23.9
作者：
Heng, Jian-Chien Dominic;Feng, Bo;Ng, Huck-Hui
通讯作者：
Ng, Huck-Hui
DOI：
10.1053/ejvs.2001.1351
发表时间：
2001-05-01
影响因子：
5.7
作者：
Danielsson, G;Albrechtsson, U;Zdanowski, Z
通讯作者：
Zdanowski, Z
DOI：
10.1161/atvbaha.109.200303
发表时间：
2010-09-01
影响因子：
8.7
作者：
Yin, Mingdi;Zhang, Jian;Xin, Shijie
通讯作者：
Xin, Shijie