When Nothing Turns Itself Inside out and Becomes Something: Coating Poly(Lactic-Co-Glycolic Acid) Spheres with Hydroxyapatite Nanoparticles vs. the Other Way Around.
When Nothing Turns Itself Inside out and Becomes Something: Coating Poly(Lactic-Co-Glycolic Acid) Spheres with Hydroxyapatite Nanoparticles vs. the Other Way Around.
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当没有东西翻过来变成什么东西时：在聚乳酸(乳酸-乙醇酸)微球上涂上羟基磷灰石纳米颗粒，反之亦然。
DOI：
10.3390/jfb13030102
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发表时间：
2022-07-23
影响因子：
4.8
通讯作者：
中科院分区：
文献类型：
--
作者：
为了稳定物理吸附在羟基磷灰石(HAP)纳米颗粒表面的药物，防止其突然释放，通常在这些纳米颗粒上包覆一层聚合物。然而，生物活性羟基磷灰石被屏蔽在这种核/壳实体的表面，这部分地违背了使用它的目的。本研究的目的是通过在聚乳酸-乙醇酸共聚物(PLGA)微球上包覆羟基磷灰石纳米颗粒来评估逆转这种经典的核/壳结构的生物学和药代动力学效应。与未修饰的PLGA微球相比，HAP壳层并不阻碍万古霉素的释放；相反，它在一定程度上提高了万古霉素的释放速率。与羟基磷灰石纳米颗粒包埋在PLGA球中的复合粒子相比，用HAP修饰PLGA球引起的矿物质沉积和成骨标志物的上调更少。这是由控制细胞代谢的动态平衡机制解释的，该机制确保细胞内部或外部的代谢产物的感觉与代谢活动的减少相一致。HAP包被的PLGA微球攻击的细胞的增殖率高于PLGA包被的HAP处理的细胞，证实了细胞增殖和骨生成之间的拮抗关系。结论是，组织对羟基磷灰石涂层生物材料的绝大多数积极反应不太可能是由于直接诱导附着在羟基磷灰石涂层的成骨细胞中的新骨生长所致。相反，这些积极的影响是细胞附着、机械转导和在羟基磷灰石涂层材料和组织之间接触处生长的更基本方面的结果。
To stabilize drugs physisorbed on the surface of hydroxyapatite (HAp) nanoparticles and prevent burst release, these nanoparticles are commonly coated with polymers. Bioactive HAp, however, becomes shielded from the surface of such core/shell entities, which partially defeats the purpose of using it. The goal of this study was to assess the biological and pharmacokinetic effects of inverting this classical core/shell structure by coating poly(lactic-co-glycolic acid) (PLGA) spheres with HAp nanoparticles. The HAp shell did not hinder the release of vancomycin; rather, it increased the release rate to a minor degree, compared to that from undecorated PLGA spheres. The decoration of PLGA spheres with HAp induced lesser mineral deposition and lesser upregulation of osteogenic markers compared to those induced by the composite particles where HAp nanoparticles were embedded inside the PLGA spheres. This was explained by homeostatic mechanisms governing the cell metabolism, which ensure than the sensation of a product of this metabolism in the cell interior or exterior is met with the reduction in the metabolic activity. The antagonistic relationship between proliferation and bone production was demonstrated by the higher proliferation rate of cells challenged with HAp-coated PLGA spheres than of those treated with PLGA-coated HAp. It is concluded that the overwhelmingly positive response of tissues to HAp-coated biomaterials for bone replacement is unlikely to be due to the direct induction of new bone growth in osteoblasts adhering to the HAp coating. Rather, these positive effects are consequential to more elementary aspects of cell attachment, mechanotransduction, and growth at the site of contact between the HAp-coated material and the tissue.
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影响因子：
9.9
作者：
Khan MA;Wu VM;Ghosh S;Uskoković V
通讯作者：
Uskoković V
DOI：
10.1002/jbm.b.33589
发表时间：
2017-04-01
影响因子：
3.4
作者：
Lee, Jae Hyup;Jang, Hae Lin;Noh, Jun Hong
通讯作者：
Noh, Jun Hong
影响因子：
4.9
作者：
Chanchareonsook, Nattharee;Tideman, Henk;Jansen, John A.
通讯作者：
Jansen, John A.
影响因子：
14
作者：
Kim, SS;Park, MS;Kim, BS
通讯作者：
Kim, BS
DOI：
10.1016/j.ejpb.2003.10.018
发表时间：
2004-03-01
影响因子：
4.9
作者：
Gavini, E;Chetoni, P;Giunchedi, P
通讯作者：
Giunchedi, P