Transcriptome sequencing and ITRAQ reveal the detoxification mechanism of Bacillus GJ1, a potential biocontrol agent for Huanglongbing.
Transcriptome sequencing and ITRAQ reveal the detoxification mechanism of Bacillus GJ1, a potential biocontrol agent for Huanglongbing.
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转录组测序和 ITRAQ 揭示黄龙病潜在生物防治剂芽孢杆菌 GJ1 的解毒机制
DOI：
10.1371/journal.pone.0200427
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发表时间：
2018
期刊：
影响因子：
3.7
通讯作者：
Jiang L
中科院分区：
文献类型：
--
作者：
Tang J;Ding Y;Nan J;Yang X;Sun L;Zhao X;Jiang L
黄龙病是世界范围内危害柑桔生产最严重的病害。没有抗HLB的柑橘品种存在。HLB病原菌亚洲黄龙病菌（Liberibacter asiaticus）是一种不可培养的病原菌，给病害的防治增加了难度。我们成功地筛选了生防剂芽孢杆菌GJ 1控制HLB在苗圃种植的柑橘植物。RNA测序（RNA-seq）的转录组和同量异序标签的相对和绝对定量的蛋白质组显示芽孢杆菌GJ 1处理和未处理的Ca的解毒反应的差异。L.感染亚洲柑橘的柑橘系统进化树分析表明，GJ 1属于B类。解淀粉酶使用实时定量聚合酶链反应（qPCR）和PCR测量消除HLB病原体的效果。结果表明，用OD 600 nm的芽孢杆菌GJ 1悬浮液灌溉7次后，植物的解毒率达到50%。最重要的是，如转录组的KEGG富集分析所示，光合作用-天线蛋白、光合作用、植物-病原体相互作用和内质网中的蛋白质加工显着上调（padj < 0.05）;九个上调的基因通过qPCR得到验证。进行转录组的转录因子分析，并通过qPCR验证10个TF。通过蛋白质组KEGG富集分析研究了氰氨基酸代谢、自噬调控、淀粉合成、淀粉和蔗糖代谢、蛋白质输出、卟啉和叶绿素代谢以及类胡萝卜素合成等途径，发现这些途径中相关基因的表达存在显著差异。蛋白质组和转录组的相关性分析分别显示了GO和KEGG途径中蛋白质表达显著不同和基因表达显著不同的共同条目。上述结果揭示了关于解毒途径的重要信息。
Huanglongbing (HLB) is the most serious disease affecting citrus production worldwide. No HLB-resistant citrus varieties exist. The HLB pathogen Candidatus Liberibacter asiaticus is nonculturable, increasing the difficulty of preventing and curing the disease. We successfully screened the biocontrol agent Bacillus GJ1 for the control of HLB in nursery-grown citrus plants. RNA sequencing (RNA-seq) of the transcriptome and isobaric tags for relative and absolute quantification of the proteome revealed differences in the detoxification responses of Bacillus GJ1-treated and -untreated Ca. L. asiaticus-infected citrus. Phylogenetic tree alignment showed that GJ1 was classified as B. amyloliquefaciens. The effect of eliminating the HLB pathogen was measured using real-time quantitative polymerase chain reaction (qPCR) and PCR. The results indicate that the rate of detoxification reached 50% after seven irrigations, of plants with an OD600nm≈1 Bacillus GJ1 suspension. Most importantly, photosynthesis-antenna proteins, photosynthesis, plant-pathogen interactions, and protein processing in the endoplasmic reticulum were significantly upregulated (padj < 0.05), as shown by the KEGG enrichment analysis of the transcriptomes; nine of the upregulated genes were validated by qPCR. Transcription factor analysis of the transcriptomes was performed, and 10 TFs were validated by qPCR. Cyanoamino acid metabolism, regulation of autophagy, isoflavonoid biosynthesis, starch and sucrose metabolism, protein export, porphyrin and chlorophyll metabolism, and carotenoid biosynthesis were investigated by KEGG enrichment analysis of the proteome, and significant differences were found in the expression of the genes involved in those pathways. Correlation analysis of the proteome and transcriptome showed common entries for the significantly different expression of proteins and the significantly different expression of genes in the GO and KEGG pathways, respectively. The above results reveal important information about the detoxification pathways.
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影响因子：
3.7
作者：
Aritua V;Achor D;Gmitter FG;Albrigo G;Wang N
通讯作者：
Wang N
DOI：
10.1073/pnas.1016981108
发表时间：
2011-05-10
影响因子：
11.1
作者：
Cao, Aizhong;Xing, Liping;Chen, Peidu
通讯作者：
Chen, Peidu
影响因子：
2.7
作者：
Gonzalez, Pedro;Reyes-De-Corcuera, Jose;Etxeberria, Ed
通讯作者：
Etxeberria, Ed
影响因子：
3.2
作者：
Chen, Xiao-Hua;Vater, Joachim;Borriss, Rainer
通讯作者：
Borriss, Rainer
影响因子：
4.1
作者：
Kalai-Grami, L.;Ben Slimane, I.;Limam, F.
通讯作者：
Limam, F.