Transcriptional and microscopic analyses of citrus stem and root responses to Candidatus Liberibacter asiaticus infection.
Transcriptional and microscopic analyses of citrus stem and root responses to Candidatus Liberibacter asiaticus infection.
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DOI：
10.1371/journal.pone.0073742
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Wang N
中科院分区：
文献类型：
--
作者：
Aritua V;Achor D;Gmitter FG;Albrigo G;Wang N
黄龙病是世界范围内危害柑桔最严重的病害。该疾病与黄龙病菌有关。HLB患病的柑橘植物发展出多种症状，包括锌和铜缺乏、斑点斑驳、木栓状脉、发育不良和小枝枯死。约L.亚洲虎的感染也严重影响根部。以往的研究主要集中在叶片和果实对Ca ~（2+）的基因表达。L.亚洲虎感染在这项研究中，我们比较了健康植物的茎和根的基因表达水平与Ca。L.利用微阵列技术检测亚洲虎感染的植物。亲和素微阵列分析显示，共有988个基因的表达发生了显著变化，其中885个在茎中，111个在根中。其中，551和56是上调，而334和55是下调的HLB病树的茎和根样品相比，健康的植物，分别。柑橘茎和根对Ca ~（2+）的转录反应存在显著差异。L.亚洲虎感染，只有8个基因在根和茎中受到影响。受影响的基因涉及多种细胞功能，包括碳水化合物代谢、细胞壁生物发生、生物和非生物胁迫反应、信号传导和转录因子、运输、细胞组织、蛋白质修饰和降解、发育、激素信号传导、金属处理和氧化还原。显微镜分析表明，淀粉的消耗在受感染的植物的根，但不是在健康的植物。细胞壁的崩溃和增厚，观察HLB影响的根，但不像在茎中严重。本研究提供了深入了解的主机响应的茎和根钙。L.亚洲虎感染
Huanglongbing (HLB) is the most destructive disease that affects citrus worldwide. The disease has been associated with Candidatus Liberibacter. HLB diseased citrus plants develop a multitude of symptoms including zinc and copper deficiencies, blotchy mottle, corky veins, stunting, and twig dieback. Ca. L. asiaticus infection also seriously affects the roots. Previous study focused on gene expression of leaves and fruit to Ca. L. asiaticus infection. In this study, we compared the gene expression levels of stems and roots of healthy plants with those in Ca. L. asiaticus infected plants using microarrays. Affymetrix microarray analysis showed a total of 988 genes were significantly altered in expression, of which 885 were in the stems, and 111 in the roots. Of these, 551 and 56 were up-regulated, while 334 and 55 were down-regulated in the stem and root samples of HLB diseased trees compared to healthy plants, respectively. Dramatic differences in the transcriptional responses were observed between citrus stems and roots to Ca. L. asiaticus infection, with only 8 genes affected in both the roots and stems. The affected genes are involved in diverse cellular functions, including carbohydrate metabolism, cell wall biogenesis, biotic and abiotic stress responses, signaling and transcriptional factors, transportation, cell organization, protein modification and degradation, development, hormone signaling, metal handling, and redox. Microscopy analysis showed the depletion of starch in the roots of the infected plants but not in healthy plants. Collapse and thickening of cell walls were observed in HLB affected roots, but not as severe as in the stems. This study provides insight into the host response of the stems and roots to Ca. L. asiaticus infection.
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影响因子：
64.5
作者：
DiLaurenzio, L;WysockaDiller, J;Benfey, PN
通讯作者：
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