Thin-film microfabricated nanofluidic arrays for size-selective protein fractionation.
Thin-film microfabricated nanofluidic arrays for size-selective protein fractionation.
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DOI：
10.1039/c3lc50869b
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发表时间：
2013-12-07
期刊：
影响因子：
6.1
通讯作者：
Woolley AT
中科院分区：
文献类型：
--
作者：
Kumar S;Xuan J;Lee ML;Tolley HD;Hawkins AR;Woolley AT
Size-selective fractionation and quantitation of biostructures in the sub-hundred nanometer size range is an important research area. Unfortunately, current methods for size fractionation are complex, time consuming, or offer poor resolution. Using standard microfabrication technology, we developed a nanofluidic sieving system to address these limitations. Our setup consists of an array of parallel nanochannels with a height step in each channel, an injection reservoir, and a waste reservoir. The height steps can size fractionate a protein mixture as a solution flows through the nanochannels via capillary action. We tested this system with different sizes and concentrations of five proteins to understand protein size and height step effects on trapping. Our results clearly show size dependent trapping of proteins at nanometer-scale height steps in nanochannels. We also developed a model that predicts the observed size-dependent trapping of proteins. This work is a key step towards scalable nanofluidic methods for molecular fractionation.
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DOI：
10.1161/01.atv.13.7.1046
发表时间：
1993-07-01
期刊：
ARTERIOSCLEROSIS AND THROMBOSIS
影响因子：
--
作者：
STEWART, MW;LAKER, MF;ALBERTI, KGMM
通讯作者：
ALBERTI, KGMM
DOI：
10.1097/iae.0b013e3181d50cea
发表时间：
2010-06-01
影响因子：
3.3
作者：
Kahook, Malik Y.;Liu, Lu;Ammar, David A.
通讯作者：
Ammar, David A.
影响因子：
4
作者：
Vinayavekhin, Nawaporn;Homan, Edwin A.;Saghatelian, Alan
通讯作者：
Saghatelian, Alan
影响因子：
6.1
作者：
Hamblin, Mark N.;Xuan, Jie;Hawkins, Aaron R.
通讯作者：
Hawkins, Aaron R.
影响因子：
10.8
作者：
Menard LD;Ramsey JM
通讯作者：
Ramsey JM