Isolation and molecular characterization of a novel homogalacturonan galacturonosyl- transferase gene (GbGAUT1) from Gossypium barbadense
Isolation and molecular characterization of a novel homogalacturonan galacturonosyl- transferase gene (GbGAUT1) from Gossypium barbadense
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海岛棉新型同型半乳糖醛酸半乳糖醛酸基转移酶基因 (GbGAUT1) 的分离和分子表征
作者：
王省芬
从海岛棉（Gossypium barbadense）中克隆了一个新基因GbGAUT 1（homogalacturonan galacturonosyltransferase gene 1）。通过生物信息学、RT-PCR和RACE等方法对海岛棉（GenBank登录号EF 552432）进行分析。该基因的开放阅读框（ORF）长1797 bp，编码598个氨基酸，预测分子量为69 kDa。GbGAUT 1是一个同源半乳糖醛酸聚糖（homogalacturonan，HG）半乳糖醛酸转移酶蛋白，具有与GAUT 1相关的超家族结构域，在系统发育树中被聚为GAUT-A组。半定量RT-PCR结果表明，GbGAUT 1基因在花后35 d的纤维中表达量最高。这些结果表明，新的棉花GbGAUT 1可能在纤维发育中发挥重要作用。
A novel gene, designated as GbGAUT1 (Gossypium barbadense homogalacturonan galacturonosyltransferase gene 1), was successfully cloned from G. barbadense (GenBank accession No. EF552432) by bioinformatics methods, RT-PCR and RACE. The open reading frame (ORF) was 1797 bp long and putatively encoded a polypeptide of 598 amino acids, with a predicted molecular mass of 69 kDa. GbGAUT1 could be considered as a homogalacturonan (HG) galacturonosyltransferase protein since the conserved GAUT1-related supperfamily motif had been found and it was clustered into GAUT-A group in the phylogenetic tree. Semi quantitative RT-PCR showed that GbGAUT1 preferentially expressed in the fiber secondary cell wall thickening, especially in the fibers of 35 day post anthesis (DPA). These results suggested that the novel cotton GbGAUT1 might play an important role in fiber development.
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