Barley SIX-ROWED SPIKE3 encodes a putative Jumonji C-type H3K9me2/me3 demethylase that represses lateral spikelet fertility.
Barley SIX-ROWED SPIKE3 encodes a putative Jumonji C-type H3K9me2/me3 demethylase that represses lateral spikelet fertility.
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DOI：
10.1038/s41467-017-00940-7
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发表时间：
2017-10-16
影响因子：
16.6
通讯作者：
Waugh R
中科院分区：
文献类型：
--
作者：
Bull H;Casao MC;Zwirek M;Flavell AJ;Thomas WTB;Guo W;Zhang R;Rapazote-Flores P;Kyriakidis S;Russell J;Druka A;McKim SM;Waugh R
大麦的花序（穗）包括一个多节的中心茎（轴），三分的单小花小穗簇沿沿着其长度交替附着。侧生小穗在每簇内的相对育性导致穗状花序具2或6排谷粒，或中间形态。了解控制这一关键发育步骤的机制可以为提高粮食产量提供新的解决方案。经典的遗传学研究确定了五个主要的六列穗（VRS）基因，其中四个现在已知编码转录因子。在这里，我们确定和保留的主要VRS基因，VRS 3，编码一个假定的Jumonji C型H3 K9 me 2/me 3去甲基化酶，染色质状态的调节器。探索由VRS 3调节的表达网络揭示了与其他VRS基因以及参与应激、激素和糖代谢的基因的特定相互作用。我们表明，结合vrs 3突变等位基因与自然的六行等位基因的VRS 1和VRS 5导致增加横向晶粒尺寸和更大的颗粒均匀性。大麦的VRS基因控制大麦花序上侧生小穗的育性。在这里，Bull等人表明VRS 3编码一种推定的Jumonji C型组蛋白脱甲基酶，该酶调节其他VRS基因以及参与应激、激素和糖代谢的基因的表达。
The barley inflorescence (spike) comprises a multi-noded central stalk (rachis) with tri-partite clusters of uni-floretted spikelets attached alternately along its length. Relative fertility of lateral spikelets within each cluster leads to spikes with two or six rows of grain, or an intermediate morphology. Understanding the mechanisms controlling this key developmental step could provide novel solutions to enhanced grain yield. Classical genetic studies identified five major SIX-ROWED SPIKE (VRS) genes, with four now known to encode transcription factors. Here we identify and characterise the remaining major VRS gene, VRS3, as encoding a putative Jumonji C-type H3K9me2/me3 demethylase, a regulator of chromatin state. Exploring the expression network modulated by VRS3 reveals specific interactions, both with other VRS genes and genes involved in stress, hormone and sugar metabolism. We show that combining a vrs3 mutant allele with natural six-rowed alleles of VRS1 and VRS5 leads to increased lateral grain size and greater grain uniformity. The VRS genes of barley control the fertility of the lateral spikelets on the barley inflorescence. Here, Bull et al. show that VRS3 encodes a putative Jumonji C-type histone demethylase that regulates expression of other VRS genes, and genes involved in stress, hormone and sugar metabolism.
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影响因子：
16.6
作者：
Cai, Qiang;Yuan, Zheng;Zhang, Dabing
通讯作者：
Zhang, Dabing
影响因子：
10.5
作者：
Chu, Zhaohui;Yuan, Meng;Wang, Shiping
通讯作者：
Wang, Shiping
影响因子：
7.4
作者：
Hooker, TS;Millar, AA;Kunst, L
通讯作者：
Kunst, L
DOI：
10.1111/j.2517-6161.1995.tb02031.x
发表时间：
1995-01-01
影响因子：
5.8
作者：
BENJAMINI, Y;HOCHBERG, Y
通讯作者：
HOCHBERG, Y
影响因子：
11.6
作者：
Fridborg, I;Kuusk, S;Sundberg, E
通讯作者：
Sundberg, E