ELISA and multiplex technologies for cytokine measurement in inflammation and aging research.
ELISA and multiplex technologies for cytokine measurement in inflammation and aging research.
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DOI：
10.1093/gerona/63.8.879
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发表时间：
2008-08
期刊：
影响因子：
--
通讯作者：
Kuchel GA
中科院分区：
文献类型：
--
作者：
Leng SX;McElhaney JE;Walston JD;Xie D;Fedarko NS;Kuchel GA
在过去的十年中，研究的重点是确定炎症在衰老，年龄相关疾病，残疾和虚弱中的作用。具有高灵敏度和特异性的测量细胞因子和其他炎症介质或标志物的方法的可用性是至关重要的。酶联免疫吸附试验（ELISA）是最广泛使用和最有效的方法，但由于其只能测量每份样品中的单一蛋白质，因此受到限制。血清细胞因子定量技术的最新发展包括多重阵列。多重阵列提供了更好地评估炎症反应的复杂性和动态性质的潜力，并提供了大量的成本和样品比传统的ELISA测量节省。尽管这种新技术具有潜在优势，但这些技术的经验有限，迄今为止尚未成为炎症介质测量的金标准。本文综述了ELISA和新兴的多重技术，比较了最近开发的多重阵列与传统ELISA技术的成本和有效性，并为有兴趣测量老年人血清炎症介质的研究人员提供了具体的建议。
Over the last decade there has been an enormous expansion of research focused on defining the role of inflammation in aging, age-related diseases, disability, and frailty. The availability of methods to measure cytokines and other inflammatory mediators or markers with high sensitivity and specificity is critically important. Enzyme-Linked Immuno-Sorbant Assay (ELISA), the most widely used and best validated method is limited by its ability to measure only a single protein in each sample. Recent developments in serum cytokine quantification technology include multiplex arrays. Multiplex Arrays offer the potential of better evaluating the complexity and dynamic nature of inflammatory responses and offer substantial cost and sample savings over traditional ELISA measurements. Despite potential advantages of this new technology, experience with these techniques is limited and it has not emerged to date as the gold standard in inflammatory mediator measurement. This article reviews ELISA and the emerging multiplex technologies, compares the cost and effectiveness of recently developed multiplex arrays with traditional ELISA technology, and provides specific recommendations for investigators interested in measuring serum inflammatory mediators in older adults.
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DOI：
10.1093/gerona/63.2.184
发表时间：
2008-02-01
影响因子：
5.1
作者：
Rudolph, James L.;Ramlawi, Basel;Marcantonio, Edward R.
通讯作者：
Marcantonio, Edward R.
DOI：
10.1186/cc5783
发表时间：
2007
期刊：
Critical care (London, England)
影响因子：
--
作者：
Bozza FA;Salluh JI;Japiassu AM;Soares M;Assis EF;Gomes RN;Bozza MT;Castro-Faria-Neto HC;Bozza PT
通讯作者：
Bozza PT
DOI：
10.1111/j.1532-5415.2007.01186.x
发表时间：
2007-06-01
影响因子：
6.3
作者：
Leng, Sean X.;Xue, Qian-Li;Fried, Linda P.
通讯作者：
Fried, Linda P.
DOI：
10.1128/cdli.6.1.89-95.1999
发表时间：
1999-01-01
期刊：
CLINICAL AND DIAGNOSTIC LABORATORY IMMUNOLOGY
影响因子：
--
作者：
Aziz, N;Nishanian, P;Fahey, JL
通讯作者：
Fahey, JL
影响因子：
5.3
作者：
Franceschi, Claudio;Capri, Miriam;Salvioli, Stefano
通讯作者：
Salvioli, Stefano