Ecdysteroid synthesis and molting by the tobacco hornworm, Manduca sexta, in the absence of prothoracic glands.
Ecdysteroid synthesis and molting by the tobacco hornworm, Manduca sexta, in the absence of prothoracic glands.
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在没有前胸腺的情况下，烟草天蛾（Manduca sexta）的蜕皮类固醇合成和蜕皮。
DOI：
10.1002/arch.940180103
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发表时间：
1991
影响因子：
2.2
通讯作者：
Gilbert,LI
中科院分区：
文献类型：
--
作者：
Sakurai,S;Warren,JT;Gilbert,LI
在化蛹当天摘除一对前胸腺后，血淋巴蜕皮激素滴度仍维持在较低水平。当一部分腺对从pupelies后prothoracotropic激素释放的关键时期，最大血淋巴蜕皮激素滴度减少的比例的前列腺素删除的质量。这些发现清楚地表明，完整的蛹中的PG负责引起按计划的成体发育所需的蜕皮激素滴度升高。当大脑在化蛹当天被移除时，成虫发育的开始被延迟了数周或数月。与此相反，蛹的PG被删除的化蛹的一天开始发展只有7天后，表明存在一个额外的来源蛹蜕皮类固醇。此外，在化蛹当天分离出的雄性六倍体成虫在70天内自发地启动了成虫发育。将0天蛹脑植入这些分离的蛹中加速了成虫发育的开始，并引起了成虫的同步发育。脑移植后5天内血淋巴蜕皮激素滴度升高，最高达1.5 μg/ml。通过反相HPLC对血淋巴蜕皮甾体的分析表明，蜕皮激素是主要部分，并且蜕皮甾体的组成与刚刚开始成体发育的正常完整蛹的组成相似。这些结果表明，PGs不是成人发育所必需的。血淋巴蜕皮激素滴度增加，也观察到孤立的睾丸被删除，并在没有消化道的睾丸。事实上，在后一种情况下，蜕皮类固醇滴度达到比那些观察到的完整的肠道寄生虫更高的水平。尽管多次尝试鉴定分离的蜕皮激素中导致蜕皮激素滴度增加的组织，但其身份仍然未知。
When a pair of prothoracic glands (PGs) were removed fromManduca sextapupae on the day of pupation, the hemolymph ecdysteroid titer remained at a low level. When a portion of the gland pair was extirpated from pupae after the critical period for prothoracicotropic hormone release, the maximum hemolymph ecdysteroid titer was reduced in proportion to the mass of the PGs removed. These findings clearly showed that the PGs in intact pupae are responsible for the elevated ecdysteroid titer required to elicit adult development on schedule. When brains were removed on the day of pupation, the initiation of adult development was delayed for weeks or months. In contrast, pupae whose PGs were removed on the day of pupation initiated development only 7 days late, indicating the existence of an additional source of pupal ecdysteroids. Further, abdomens of maleM.sextathat were isolated on the day of pupation initiated adult development spontaneously within 70 days. The implantation of day 0 pupal brains into these isolated abdomens accelerated the initiation of adult development and elicited synchronous adult development. The hemolymph ecdysteroid titer of those isolated abdomens receiving implants of brains increased within 5 days and reached a maximum level of 1.5 μg/ml. The analysis of hemolymph ecdysteroids by reverse‐phase HPLC revealed that ecdysone was the major moiety and that the ecdysteroid composition was similar to that of normal, intact pupae that had just initiated adult development. These results demonstrate that the PGs are not requisite for adult development. An increased hemolymph ecdysteroid titer was also observed in isolated abdomens from which the testes were removed and in abdomens devoid of their digestive tract. Indeed, in the latter case, the ecdysteroid titer attained much higher levels than those observed for abdomens with intact guts. Despite numerous attempts to identity the tissue(s) in the isolated abdomens responsible for the increase in ecdysteroid titer, its identity remains unknown.
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DOI：
--
发表时间：
1974
期刊：
影响因子：
--
作者：
D. S. King;W. Bollenbacher;D. Borst;W. Vedeckis;J. D. O'connor;P. I. Ittycheriah;L. Gilbert
通讯作者：
L. Gilbert
烟草天蛾（Manduca Sexta）颈部和腹部结扎蛹制剂中滞育的提前终止
DOI：
--
发表时间：
1986
期刊：
影响因子：
--
作者：
L. Safranek;C. R. Squire;Carroll M. Williams
通讯作者：
Carroll M. Williams
鳞翅目幼虫和蛹蜕皮激素滴度波动的调节及意义
DOI：
--
发表时间：
1981
期刊：
影响因子：
--
作者：
F. Sehnal;P. Maróy;J. Malá
通讯作者：
J. Malá
烟草芽虫幼虫睾丸：昆虫蜕皮类固醇的新来源
影响因子：
56.9
作者：
M. Loeb;C. W. Woods;Ethel P. Brandt;Alexej B. Boakovec
通讯作者：
Alexej B. Boakovec
烟草天蛾游走行为的生理学。
DOI：
10.1242/jeb.117.1.45
发表时间：
1985
期刊：
The Journal of experimental biology
影响因子：
--
作者：
Dominick,OS;Truman,JW
通讯作者：
Truman,JW