Regulation of FADS2 transcription by SREBP-1 and PPAR-α influences LC-PUFA biosynthesis in fish.
Regulation of FADS2 transcription by SREBP-1 and PPAR-α influences LC-PUFA biosynthesis in fish.
复制标题
SREBP-1 和 PPAR-α 调节 FADS2 转录影响鱼类 LC-PUFA 生物合成
DOI：
10.1038/srep40024
复制
发表时间：
2017-01-09
影响因子：
4.6
通讯作者：
Ai Q
中科院分区：
文献类型：
--
作者：
Dong X;Tan P;Cai Z;Xu H;Li J;Ren W;Xu H;Zuo R;Zhou J;Mai K;Ai Q
本研究旨在探讨鱼类生物合成长链多不饱和脂肪酸（LC-PUFAs）能力差异的机制。用植物油替代鱼油后，虹鳟鱼、鲈鱼和大黄鱼肌肉和肝脏中18碳脂肪酸含量不同程度增加，n-3 LC-PUFA含量降低，表明这3种鱼生物合成LC-PUFA的能力不同。鱼油替代还导致FADS 2和SREBP-1在三种鱼肝脏中的表达显著上调，但两种PPAR-α同源物的反应不同。体外实验表明，虹鳟鱼FADS 2启动子的基本转录活性显著高于日本鲈鱼和大黄鱼，这与它们的LC-PUFA生物合成能力相一致。此外，SREBP-1和PPAR-α上调FADS 2启动子活性。这些调节作用在SREBP-1和PPAR-α之间以及三种鱼之间存在很大差异。综上所述，这两种转录因子对FADS 2的调控活性的差异可能是这三种鱼适应不同环境盐度的不同LC-PUFA生物合成能力的原因。
The present study was conducted to explore the mechanisms leading to differences among fishes in the ability to biosynthesize long-chain polyunsaturated fatty acids (LC-PUFAs). Replacement of fish oil with vegetable oil caused varied degrees of increase in 18-carbon fatty acid content and decrease in n-3 LC-PUFA content in the muscle and liver of rainbow trout (Oncorhynchus mykiss), Japanese seabass (Lateolabrax japonicus) and large yellow croaker (Larimichthys crocea), suggesting that these fishes have differing abilities to biosynthesize LC-PUFAs. Fish oil replacement also led to significantly up-regulated expression of FADS2 and SREBP-1 but different responses of the two PPAR-α homologues in the livers of these three fishes. An in vitro experiment indicated that the basic transcription activity of the FADS2 promoter was significantly higher in rainbow trout than in Japanese seabass or large yellow croaker, which was consistent with their LC-PUFA biosynthetic abilities. In addition, SREBP-1 and PPAR-α up-regulated FADS2 promoter activity. These regulatory effects varied considerably between SREBP-1 and PPAR-α, as well as among the three fishes. Taken together, the differences in regulatory activities of the two transcription factors targeting FADS2 may be responsible for the different LC-PUFA biosynthetic abilities in these three fishes that have adapted to different ambient salinity.
登录
查看更多内容
影响因子：
2.7
作者：
Cho, Hyun Kook;Kong, Hee Jeong;Cheong, JaeHun
通讯作者：
Cheong, JaeHun
DOI：
10.1016/j.bbrc.2008.01.075
发表时间：
2008-04-04
影响因子：
3.1
作者：
Kumadaki, Shin;Matsuzaka, Takashi;Shimano, Hitoshi
通讯作者：
Shimano, Hitoshi
影响因子：
4.5
作者：
Codabaccus, Basseer M.;Bridle, Andrew R.;Carter, Chris G.
通讯作者：
Carter, Chris G.
影响因子：
4.2
作者：
Bell, JG;McEvoy, J;Sargent, JR
通讯作者：
Sargent, JR
DOI：
10.1016/s1096-4959(03)00143-x
发表时间：
2003-08-01
影响因子：
2.2
作者：
Bransden, MP;Carter, CG;Nichols, PD
通讯作者：
Nichols, PD