Alcohol induced alterations to the human fecal VOC metabolome.
Alcohol induced alterations to the human fecal VOC metabolome.
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DOI：
10.1371/journal.pone.0119362
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发表时间：
2015
期刊：
影响因子：
3.7
通讯作者：
Keshavarzian A
中科院分区：
文献类型：
--
作者：
Couch RD;Dailey A;Zaidi F;Navarro K;Forsyth CB;Mutlu E;Engen PA;Keshavarzian A
研究表明，过量饮酒会影响肠道微生物群的组成，导致体内平衡的破坏（生态失调）。然而，这种观察到的变化并不表明可能导致有害和有毒产品产生的肠道微生物群功能失调。因此，有必要了解酒精对肠道微生物群功能及其代谢产物的影响，以便更好地了解肠道微生物群在酒精相关器官衰竭中的作用。在这里，我们报告的差异代谢组学分析的结果比较挥发性有机化合物（VOC）的酗酒者和非酒精健康对照组的粪便中检测到的。我们对传代后采集的粪便样本以及直接从乙状结肠腔采集的样本进行了分析。不管粪便收集的方法，我们发现了一个粪便VOC代谢组学签名酗酒者是不同于健康对照。酒精样品中最显著的代谢物改变包括：（1）氧化应激生物标志物十四烷的升高;（2）具有抗氧化性质的五种脂肪醇的减少;（3）短链脂肪酸丙酸酯和异丁酸酯的减少，这对维持肠上皮细胞健康和屏障完整性很重要;（4）酒精消耗天然抑制剂亚麻酸的减少;（5）脂肪酸的减少。（5）天然产物和肝脂肪变性衰减剂降冰片烯的减少;和（6）二甲基二硫和二甲基三硫的减少，二甲基二硫和二甲基三硫是分解的微生物产物。我们的研究结果表明，酗酒者的肠道微生物群功能发生了改变，这可能会促进酒精相关的病理。
Studies have shown that excessive alcohol consumption impacts the intestinal microbiota composition, causing disruption of homeostasis (dysbiosis). However, this observed change is not indicative of the dysbiotic intestinal microbiota function that could result in the production of injurious and toxic products. Thus, knowledge of the effects of alcohol on the intestinal microbiota function and their metabolites is warranted, in order to better understand the role of the intestinal microbiota in alcohol associated organ failure. Here, we report the results of a differential metabolomic analysis comparing volatile organic compounds (VOC) detected in the stool of alcoholics and non-alcoholic healthy controls. We performed the analysis with fecal samples collected after passage as well as with samples collected directly from the sigmoid lumen. Regardless of the approach to fecal collection, we found a stool VOC metabolomic signature in alcoholics that is different from healthy controls. The most notable metabolite alterations in the alcoholic samples include: (1) an elevation in the oxidative stress biomarker tetradecane; (2) a decrease in five fatty alcohols with anti-oxidant property; (3) a decrease in the short chain fatty acids propionate and isobutyrate, important in maintaining intestinal epithelial cell health and barrier integrity; (4) a decrease in alcohol consumption natural suppressant caryophyllene; (5) a decrease in natural product and hepatic steatosis attenuator camphene; and (6) decreased dimethyl disulfide and dimethyl trisulfide, microbial products of decomposition. Our results showed that intestinal microbiota function is altered in alcoholics which might promote alcohol associated pathologies.
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DOI：
10.1016/j.alcohol.2008.12.009
发表时间：
2009-03
期刊：
Alcohol (Fayetteville, N.Y.)
影响因子：
--
作者：
Forsyth CB;Farhadi A;Jakate SM;Tang Y;Shaikh M;Keshavarzian A
通讯作者：
Keshavarzian A
DOI：
10.1016/j.trstmh.2009.02.004
发表时间：
2009-11-01
影响因子：
2.2
作者：
Garner, C. E.;Smith, S.;Probert, C. S. J.
通讯作者：
Probert, C. S. J.
影响因子：
4.1
作者：
Bos, R;Koulman, A;Pras, N
通讯作者：
Pras, N
影响因子：
3.8
作者：
Costello, B. De Lacy;Ewen, R.;Smith, S.
通讯作者：
Smith, S.
影响因子：
3.7
作者：
Bull-Otterson L;Feng W;Kirpich I;Wang Y;Qin X;Liu Y;Gobejishvili L;Joshi-Barve S;Ayvaz T;Petrosino J;Kong M;Barker D;McClain C;Barve S
通讯作者：
Barve S