Overexpression of the tissue inhibitor of metalloproteinase-3 during Xenopus embryogenesis affects head and axial tissue formation
Overexpression of the tissue inhibitor of metalloproteinase-3 during Xenopus embryogenesis affects head and axial tissue formation
复制标题
非洲爪蟾胚胎发生过程中金属蛋白酶-3组织抑制剂的过度表达影响头部和轴向组织的形成
作者：
B. Pickard;S. Damjanovski
摘要金属蛋白酶组织抑制剂（TIMP）在胚胎发育和疾病过程中调节细胞外基质重塑。在非洲爪蟾胚胎发生过程中检查了 TIMP-3 的表达：在母体 RNA 库中检测到的 TIMP-3 转录本在囊胚中期转变时增加，在原肠胚形成期间急剧下降，并在神经形成和轴伸长期间再次增加。有趣的是，在氯化锂处理（背侧化）胚胎中没有看到原肠胚形成过程中的减少。使用 DIG 标记的 RNA 探针对 TIMP-3 进行整体原位杂交，证明转录物在胚胎发生期间存在于所有背部组织中，但仅在从第 35 阶段开始的头部结构中突出。通过转基因和 RNA 注射过度表达 TIMP-3 会导致发育异常和死亡。两种过度表达策略都会导致原肠胚形成后的扰动，包括对神经和头部结构以及截短轴的扰动。然而，RNA注射导致更严重的早期缺陷，例如神经管闭合失败，转基因导致轴截短和头部异常。表达 TIMP-3 的转基因胚胎没有存活到第 40 阶段。
ABSTRACTTissue inhibitors of metalloproteinases (TIMPs) modulate extracellular matrix remodeling during embryonic development and disease. TIMP-3 expression was examined during Xenopus laevis embryogenesis: TIMP-3 transcripts detected in the maternal pool of RNA increased at the mid-blastula transition, decreased dramatically during gastrulation and increased again during neurulation and axis elongation. Interestingly, the decrease during gastrulation was not seen in LiCl treated (dorsalized) embryos. Whole mount in situ hybridization of TIMP-3 using DIG-labeled RNA probes demonstrated that the transcripts were present in all dorsal tissues during embryogenesis, but were prominent only in head structures starting at stage 35. Overexpression of TIMP-3 through transgenesis and RNA injections led to developmental abnormalities and death. Both overexpression strategies resulted in post-gastrulation perturbation including those to neural and head structures, as well as truncated axes. However, RNA injections resulted in more severe early defects such as failure of neural tube closure, and transgenesis caused truncated axes and head abnormalities. No transgenic embryo expressing TIMP-3 survived past stage 40.
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DOI：
10.1016/s0021-9258(17)37115-6
发表时间：
1994-03
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
K. Leco;R. Khokha;N. Pavloff;S. Hawkes;D. Edwards
通讯作者：
K. Leco;R. Khokha;N. Pavloff;S. Hawkes;D. Edwards
DOI：
10.1152/ajpheart.00511.2002
发表时间：
2003-01-01
影响因子：
4.8
作者：
Creemers, EEJM;Davis, JN;Spinale, FG
通讯作者：
Spinale, FG
编码大鼠组织金属蛋白酶抑制剂 3 (TIMP-3) 的 cDNA 的克隆和表达。
影响因子：
3.5
作者：
Wu,I;Moses,MA
通讯作者：
Moses,MA
DOI：
10.1016/s0021-9258(18)41928-x
发表时间：
1992-08
期刊：
The Journal of biological chemistry
影响因子：
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作者：
N. Pavloff;P. Staskus;N. Kishnani;Susan P. HawkesQ
通讯作者：
N. Pavloff;P. Staskus;N. Kishnani;Susan P. HawkesQ
胚胎晶状体诱导：超过满足视神经泡。
DOI：
10.1016/0922-3371(89)90001-4
发表时间：
1989
期刊：
Cell differentiation and development : the official journal of the International Society of Developmental Biologists
影响因子：
--
作者：
Saha,MS;Spann,CL;Grainger,RM
通讯作者：
Grainger,RM