Sox2 is required for maintenance and regeneration, but not initial development, of hair cells in the zebrafish inner ear.
Sox2 is required for maintenance and regeneration, but not initial development, of hair cells in the zebrafish inner ear.
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DOI：
10.1016/j.ydbio.2009.12.011
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发表时间：
2010-02-15
影响因子：
2.7
通讯作者：
Riley BB
中科院分区：
文献类型：
--
作者：
Millimaki BB;Sweet EM;Riley BB
Sox2与多能干细胞的维持或细胞分化的早期阶段有不同的关系，这取决于上下文。在发育中的内耳中，Sox2最初标记新生感觉上皮中的所有细胞，并且在小鼠中，它是感觉上皮形成所必需的。Sox2最终在毛细胞中下调，但在支持细胞中维持，其功能意义尚不清楚。本文描述了sox2在斑马鱼内耳中的调控和功能。sox2在感觉上皮发育开始后开始表达，受Atoh1a/b、Fgf和Notch的调控。sox2基因的敲除并不会阻止毛细胞的产生，但由于分化的毛细胞零星死亡，其积累速度会降低。接下来，我们测试了激光消融成熟brn3c:gfp标记的毛细胞后毛细胞再生的能力。在对照胚胎中，丢失的毛细胞在消融后12小时开始再生，涉及支持细胞的转分化，而不是不对称的细胞分裂。相反，在sox2耗尽的胚胎中不会发生再生。这些数据表明，斑马鱼的毛细胞存活以及再生过程中支持细胞向毛细胞的转分化都需要sox2。
Sox2 has been variously implicated in maintenance of pluripotent stem cells or, alternatively, early stages of cell differentiation, depending on context. In the developing inner ear, Sox2 initially marks all cells in the nascent sensory epithelium and, in mouse, is required for sensory epithelium formation. Sox2 is eventually downregulated in hair cells but is maintained in support cells, the functional significance of which is unknown. Here we describe regulation and function of sox2 in the zebrafish inner ear. Expression of sox2 begins after the onset of sensory epithelium development and is regulated by Atoh1a/b, Fgf and Notch. Knockdown of sox2 does not prevent hair cell production, but the rate of accumulation is reduced due to sporadic death of differentiated hair cells. We next tested the capacity for hair cell regeneration following laser-ablation of mature brn3c:gfp-labeled hair cells. In control embryos, regeneration of lost hair cells begins by 12 hours post-ablation and involves transdifferentiation of support cells rather than asymmetric cell division. In contrast, regeneration does not occur in sox2-depleted embryos. These data show that zebrafish sox2 is required for hair cell survival, as well as for transdifferentiation of support cells into hair cells during regeneration.
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影响因子：
5.7
作者：
Edge AS;Chen ZY
通讯作者：
Chen ZY
影响因子：
2.7
作者：
Gray, M;Moens, CB;Beattie, CE
通讯作者：
Beattie, CE
DOI：
10.1073/pnas.0808175105
发表时间：
2008-11-25
影响因子：
11.1
作者：
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通讯作者：
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影响因子：
16.2
作者：
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通讯作者：
Partanen, J
影响因子：
2.7
作者：
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通讯作者：
Hamre, Kristin M.