In vitro replication and cytopathogenicity of the feline immunodeficiency virus for feline T4 thymic lymphoma 3201 cells.
In vitro replication and cytopathogenicity of the feline immunodeficiency virus for feline T4 thymic lymphoma 3201 cells.
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猫免疫缺陷病毒对猫 T4 胸腺淋巴瘤 3201 细胞的体外复制和细胞致病性。
DOI：
10.1016/0042-6822(90)90323-j
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发表时间：
1990
期刊：
影响因子：
3.7
通讯作者：
Olsen,RG
中科院分区：
文献类型：
--
作者：
Tochikura,TS;Hayes,KA;Cheney,CM;Tanabe-Tochikura,A;Rojko,JL;Mathes,LE;Olsen,RG
用猫免疫缺陷病毒的Petaluma分离株（FIV-Petaluma）在猫T4胸腺淋巴瘤3201细胞中建立了细胞毒性猫免疫缺陷病毒（FIV）感染。Mg 2+依赖性逆转录酶（Mg 2 +RT）活性和FIV p24/28阳性细胞在接种后18天（dpi）开始明显。在22 dpi开始观察到细胞死亡，最多40%死亡（在26 dpi台盼蓝染料排除）。在培养的Crandall猫肾成纤维细胞中未观察到类似的FIV-Petaluma感染的这种杀细胞变化。存活的细胞生长出来，并建立了慢性FIV生产细胞系。3201细胞衍生的FIV（FIV-3201）对于FIV-未处理的猫3201细胞毒性更强，FIV p24/28-阳性细胞和Mg 2 +RT活性首先在4-8 dpi可检测到，随后在8-12 dpi可检测到细胞活力丧失。在16 dpi时观察到最大杀灭（40%死亡）。FIV-Petaluma和FIV-3201对FIV-初始3201细胞的病毒感染性之间的比较显示，通过在3201细胞中扩增/传代，组织培养感染剂量（TCID 50）增加1 log 10。FIV感染培养物中3201细胞的细胞学和电子显微镜检查显示频繁出芽的慢病毒颗粒。这种裂解性感染系统为FIV感染猫的FIV的常规检测、分离和定量、病毒的大规模繁殖以及用于评价FIV淋巴细胞毒性机制和开发对抗慢病毒细胞病变的疗法的系统开辟了道路。
Cytotoxic feline immunodeficiency virus (FIV) infection was established in feline T4 thymic lymphoma 3201 cells with the Petaluma isolate of the feline immunodeficiency virus (FIV-Petaluma). Mg2+-dependent, reverse transcriptase (Mg2+RT) activity and FIV p24/28-positive cells were evident beginning at 18 days postinoculation (dpi). Cell death was observed beginning at 22 dpi, with a maximum of 40% dead (trypan blue dye exclusion at 26 dpi). This cytocidal change was not observed in cultured Crandall feline kidney fibroblasts similarly infected with FIV-Petaluma. The surviving cells grew out and a chronic FIV-producer cell line was established. The 3201 cell-derived FIV (FIV-3201) was far more virulent for FIV-naive feline 3201 cells, with FIV p24/28-positive cells and Mg2+RT activity first detectable by 4–8 dpi and subsequent loss of cell viability detectable by 8–12 dpi. Maximum kill (40% dead) was observed at 16 dpi. Comparison between viral infectivity of FIV-Petaluma and FIV-3201 for FIV-naive 3201 cells showed an increase of 1 log10tissue culture infectious doses (TCID50) by amplification/passage in 3201 cells. Cytologic and electron microscopic examination of 3201 cells in FIV-infected cultures showed frequent budding lentiviral particles. This lytic infection system opens the way to the routine detection, isolation, and quantitation of FIV from FIV-infected cats, to the large-scale propagation of the virus, and to a system for evaluation of the mechanisms of FIV lymphocytotoxicity and the development of therapies to counteract lentiviral cytopathicity.
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作者：
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--
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期刊：
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