In situ, in vivo, and in vitro approaches for studying AMR plasmid conjugation in the gut microbiome.
In situ, in vivo, and in vitro approaches for studying AMR plasmid conjugation in the gut microbiome.
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DOI：
10.1093/femsre/fuac044
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发表时间：
2023-01-16
影响因子：
11.3
通讯作者：
中科院分区：
文献类型：
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作者：
抗菌素耐药性(AMR)是一个全球性的威胁，一线抗生素耐药性的演变和传播速度超过了新治疗方法的发展。AMR的传播是通过在细菌之间转移抗菌素耐药基因(ARGs)来实现的，尤其是那些由接合质粒编码的基因。人类肠道微生物组是已知的质粒接合的‘大熔炉’，ARG在这种环境中的转移被广泛地记录下来。有必要更好地了解影响这些转移事件发生的因素，并研究潜在地对抗ARGs传播的方法。该综述描述了三种研究人类肠道结合的方法的应用和潜力：住院患者的原位事件观察，以啮齿动物模型为主的体内微生物组的模拟，以及各种复杂性的体外模型的使用。每一种都为我们理解肠道中的共轭带来了独特的见解。这些系统及其组合的使用和开发将对更好地理解肠道微生物组中水平基因转移的重要性、普遍性和可操作性至关重要。讨论了原位、体内和体外方法，用于了解抗菌素耐药性是如何通过在人类肠道微生物群中的细菌之间的接合而传播的。
Antimicrobial resistance (AMR) is a global threat, with evolution and spread of resistance to frontline antibiotics outpacing the development of novel treatments. The spread of AMR is perpetuated by transfer of antimicrobial resistance genes (ARGs) between bacteria, notably those encoded by conjugative plasmids. The human gut microbiome is a known ‘melting pot’ for plasmid conjugation, with ARG transfer in this environment widely documented. There is a need to better understand the factors affecting the incidence of these transfer events, and to investigate methods of potentially counteracting the spread of ARGs. This review describes the use and potential of three approaches to studying conjugation in the human gut: observation of in situ events in hospitalized patients, modelling of the microbiome in vivo predominantly in rodent models, and the use of in vitro models of various complexities. Each has brought unique insights to our understanding of conjugation in the gut. The use and development of these systems, and combinations thereof, will be pivotal in better understanding the significance, prevalence, and manipulability of horizontal gene transfer in the gut microbiome. A discussion of the in situ, in vivo and in vitro approaches used to understand how antimicrobial resistance spreads by conjugation between bacteria in the human gut microbiome.
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DOI：
10.1098/rspb.2021.2027
发表时间：
2021-11-10
期刊：
Proceedings. Biological sciences
影响因子：
--
作者：
Duxbury SJN;Alderliesten JB;Zwart MP;Stegeman A;Fischer EAJ;de Visser JAGM
通讯作者：
de Visser JAGM
影响因子：
3.2
作者：
Aghamohammad, Shadi;Badmasti, Farzad;Shahcheraghi, Fereshteh
通讯作者：
Shahcheraghi, Fereshteh
DOI：
10.1073/pnas.2003407117
发表时间：
2020-10-06
影响因子：
11.1
作者：
David S;Cohen V;Reuter S;Sheppard AE;Giani T;Parkhill J;European Survey of Carbapenemase-Producing Enterobacteriaceae (EuSCAPE) Working Group;ESCMID Study Group for Epidemiological Markers (ESGEM);Rossolini GM;Feil EJ;Grundmann H;Aanensen DM
通讯作者：
Aanensen DM
影响因子：
6.4
作者：
Aviv G;Rahav G;Gal-Mor O
通讯作者：
Gal-Mor O
影响因子：
64.8
作者：
ANDERSON, ES
通讯作者：
ANDERSON, ES