Comparison of chondrogenesis-related biological behaviors between human urine-derived stem cells and human bone marrow mesenchymal stem cells from the same individual.
Comparison of chondrogenesis-related biological behaviors between human urine-derived stem cells and human bone marrow mesenchymal stem cells from the same individual.
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DOI：
10.1186/s13287-021-02370-1
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发表时间：
2021-06-28
影响因子：
7.5
通讯作者：
Xiang Z
中科院分区：
文献类型：
--
作者：
Sun J;Xing F;Zou M;Gong M;Li L;Xiang Z
干细胞是组织工程种子细胞的主要选择，但使用大多数传统干细胞需要侵入性和复杂的程序。人尿源性干细胞（hUSCs）是一种替代性干细胞来源，具有非侵入性和重复性分离自同一个体的优点。本研究的目的是比较同一个体的人USCs和人骨髓间充质干细胞（hBMSCs）的软骨形成相关的生物学行为。hUSCs和hBMSCs分离自6名接受髂骨移植的患者。在体外进行细胞形态、增殖、集落形成、迁移和多分化分析。然后，制备脱细胞软骨细胞外基质（ACM）支架用于体内植入。在植入前比较hUSCs和hBMSCs在支架上培养的细胞活力、形态、增殖和软骨形成。将负载hUSCs或hBMSCs的支架植入兔膝关节模型中修复软骨缺损。通过磁共振成像（MRI）和显微计算机断层扫描（μCT）分析、炎症和毒性试验、大体观察和组织学评价来评价软骨修复效果。在体外实验中，hUSCs具有更好的增殖能力，集落形成，和迁移相比，在同一代hBMSCs，而hBMSCs有更大的成骨，成脂，成软骨能力相比，在同一代hUSCs。与其他传代细胞相比，第3代hUSCs和hBMSCs具有最强的增殖、集落形成和多系分化潜力。植入后12周，hUSCs和hBMSCs负载的ACM支架均能显著促进兔膝关节软骨缺损的修复，新生组织以透明软骨为主。然而，hUSCs和hBMSCs之间的软骨修复效果没有显著差异。在体外实验中，hUSCs表现出更好的增殖能力，而hBMSCs具有更强的软骨形成能力。然而，hUSCs和hBMSCs在体内具有相似的软骨修复作用。结果表明，hUSCs可以作为软骨再生的干细胞替代物，并为软骨组织工程和临床转化提供了强有力的平台。在线版本包含补充材料，可通过10.1186/s13287-021-02370-1获得。
Stem cells are the main choice for seed cells in tissue engineering, but using most traditional stem cells requires invasive and complicated procedures. Human urine-derived stem cells (hUSCs) are an alternative stem cell source with the advantages of being isolated noninvasively and repetitively from the same individual. The aim of this study was to compare chondrogenesis-related biological behaviors between hUSCs and human bone marrow mesenchymal stem cells (hBMSCs) from the same individual. hUSCs and hBMSCs were isolated from six patients who underwent iliac bone grafting. Cell morphology, proliferation, colony-forming, migration, and multidifferentiation analyses were performed in vitro. Then, acellular cartilage extracellular matrix (ACM) scaffolds were fabricated for in vivo implantation. The comparisons of cell viability, morphology, proliferation, and chondrogenesis between hUSCs and hBMSCs cultured on scaffolds were performed before implantation. The scaffolds loaded with hUSCs or hBMSCs were implanted into a rabbit knee model to repair cartilage defects. Magnetic resonance imaging (MRI) and micro-computed tomography (μCT) Analyses, inflammation and toxicity assays, gross observation, and histological evaluation were performed to evaluate the cartilage repair effects. In in vitro experiments, hUSCs had better capacity for proliferation, colony-forming, and migration compared to hBMSCs in the same passage, while hBMSCs had greater osteogenic, adipogenic, and chondrogenic abilities compared to hUSCs in the same passage. Both hUSCs and hBMSCs at passage 3 had the strongest potential for proliferation, colony-forming, and multilineage differentiation compared to cells in other passages. The ACM scaffolds loaded with hUSCs or hBMSCs both significantly promoted the repair of cartilage defects in the rabbit knee model at 12 weeks’ postimplantation, and the new tissue was mainly hyaline cartilage. However, there was no significant difference in cartilage repair effects between hUSCs and hBMSCs. In in vitro experiments, hUSCs presented better capacity for proliferation, while hBMSCs had greater chondrogenic ability. However, hUSCs and hBMSCs had similar cartilage repair effects in vivo. Results indicated that hUSCs can be a stem cell alternative for cartilage regeneration and provide a powerful platform for cartilage tissue engineering and clinical transformation. The online version contains supplementary material available at 10.1186/s13287-021-02370-1.
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