Biofilm-Forming Capacity and Drug Resistance of Different Gardnerella Subgroups Associated with Bacterial Vaginosis.
Biofilm-Forming Capacity and Drug Resistance of Different Gardnerella Subgroups Associated with Bacterial Vaginosis.
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DOI：
10.3390/microorganisms11092186
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发表时间：
2023-08-30
期刊：
影响因子：
4.5
通讯作者：
Xiao B
中科院分区：
文献类型：
--
作者：
Qin H;Liu Y;Zhai Z;Xiao B
细菌性阴道炎（BV）是育龄女性下生殖道最常见的感染疾病。与生物膜相关的复发性感染和抗生素耐药性，仍是BV治疗面临的重大挑战。加德纳菌属在患与未患BV的女性中均常见，这表明加德纳菌分离株之间的遗传差异或许能区分致病性亚群和共生性亚群。本研究从接受治疗前后的BV女性阴道样本中分离出11株加德纳菌。通过结晶紫染色检测每株菌的生物膜形成能力。利用cpn60序列的系统发育分析筛选出8株菌，并将其分为A亚群（6/8）、B亚群（1/8）和D亚群（1/8）。比较这些菌株在不同亚群间的生物膜形成能力和抗生素耐药谱。D亚群的生物膜形成能力最强。6株浮游菌株对BV一线治疗药物甲硝唑表现出耐药性，1株对克林霉素耐药。此外，体外生物膜形成增加了菌株对克林霉素的耐药性。选取2株生物膜形成能力强的菌株S20和S23，以及2株生物膜形成能力弱的菌株S24和S25进行比较基因组分析。研究发现，与S24和S25相比，S20和S23含有4个与生物膜形成相关的关键基因，以及更多参与碳水化合物合成与代谢的基因。明确加德纳菌不同亚群间毒力因子表达的差异，可为开发BV的新型治疗方法提供参考。
Bacterial vaginosis (BV) is the most common infection of the lower reproductive tract among women of reproductive age. Recurrent infections and antibiotic resistance associated with biofilms remain significant challenges for BV treatment. Gardnerella species are commonly found in women with and without BV, indicating that genetic differences among Gardnerella isolates may distinguish pathogenic from commensal subgroups. This study isolated 11 Gardnerella strains from vaginal samples obtained from women with BV before or after treatment. The biofilm formation ability of each strain was examined by crystal violet staining. Eight strains were selected using phylogenetic analysis of the cpn60 sequences and classified as subgroups A (6/8), B (1/8), and D (1/8). The biofilm formation ability and antibiotic resistance profile of these strains was compared among the subgroups. Subgroup D had the strongest biofilm formation ability. Six of the planktonic strains exhibited resistance to the first-line BV drug, metronidazole, and one to clindamycin. Moreover, biofilm formation in vitro increased strain resistance to clindamycin. Two strains with strong biofilm ability, S20 and S23, and two with weak biofilm ability, S24 and S25, were selected for comparative genomic analysis. S20 and S23 were found to contain four key genes associated with biofilm formation and more genes involved in carbohydrate synthesis and metabolism than S24 and S25. Identifying differences in the expression of virulence factors between Gardnerella subgroups could inform the development of novel treatments for BV.
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