Effect-directed analysis of Elizabeth River porewater: developmental toxicity in zebrafish (Danio rerio).
Effect-directed analysis of Elizabeth River porewater: developmental toxicity in zebrafish (Danio rerio).
复制标题
伊丽莎白河河谷的效应指导分析：斑马鱼的发育毒性（Danio Rerio）。
DOI：
10.1002/etc.2738
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发表时间：
2014-12
影响因子：
4.1
通讯作者：
Stapleton, Heather M.
中科院分区：
文献类型：
--
作者：
Fang, Mingliang;Getzinger, Gordon J.;Cooper, Ellen M.;Clark, Bryan W.;Garner, Lindsey V. T.;Di Giulio, Richard T.;Ferguson, P. Lee;Stapleton, Heather M.
关键词：
在本研究中，效应导向分析被用来确定致畸化合物的孔隙水收集从超级基金网站沿着伊丽莎白河河口（VA，美国）。斑马鱼（Danio rerio）暴露于孔隙水显示急性发育毒性和心脏畸形，可能是因为历史上使用杂酚油沉积物中多环芳烃（PAHs）水平升高。用几种物理和化学颗粒去除方法对孔隙水进行预处理，发现胶体结合的化学品构成了所观察到的毒性的大部分。采用尺寸排阻色谱法和正相高效液相色谱法对伊丽莎白河孔隙水进行了分离。急性毒性孔隙水提取物和提取物馏分评估为胚胎斑马鱼的心包区。毒性最大的部分含有几种已知的芳烃受体（AhR）激动剂（例如，1，2-苯并芴和1，2-苯并蒽）和细胞色素P450 A1（CPY 1A）抑制剂（例如，二苯并噻吩和荧蒽）。毒性第二大的级分含有已知的AhR激动剂（例如，苯并[a]芘和茚并[1，2，3-cd]芘）。添加CYP 1A抑制剂，荧蒽，增加所有活性孔隙水组分的毒性，表明存在于孔隙水中的几种污染物之间的协同作用。结果表明，观察到的急性毒性与伊丽莎白河孔隙水的结果从高浓度的AhR激动多环芳烃和混合物的影响有关的化合物之间的相互作用，共同发生在伊丽莎白河网站。然而，即使经过广泛的分馏和化学表征，它仍然是合理的，在伊丽莎白河孔隙水中的一些活性化合物仍然不明。
In the present study, effect-directed analysis was used to identify teratogenic compounds in porewater collected from a Superfund site along the Elizabeth River estuary (VA, USA). Zebrafish (Danio rerio) exposed to the porewater displayed acute developmental toxicity and cardiac teratogenesis, presumably because of elevated sediment levels of polycyclic aromatic hydrocarbons (PAHs) from historical creosote use. Pretreatment of porewater with several physical and chemical particle removal methods revealed that colloid-bound chemicals constituted the bulk of the observed toxicity. Size-exclusive chromatography and normal-phase high-performance liquid chromatography were used to fractionate Elizabeth River porewater. Acute toxicity of porewater extracts and extract fractions was assessed as the pericardial area in embryonic zebrafish. The most toxic fraction contained several known aryl hydrocarbon receptor (AhR) agonists (e.g., 1,2-benzofluorene and 1,2-benzanthracene) and cytochrome P450 A1 (CPY1A) inhibitors (e.g., dibenzothiophene and fluoranthene). The second most toxic fraction contained known AhR agonists (e.g., benzo[a]pyrene and indeno[1,2,3-cd]pyrene). Addition of a CYP1A inhibitor, fluoranthene, increased toxicity in all active porewater fractions, suggesting synergism between several contaminants present in porewaters. The results indicate that the observed acute toxicity associated with Elizabeth River porewater results from high concentrations of AhR agonistic PAHs and mixture effects related to interactions between compounds co-occurring at the Elizabeth River site. However, even after extensive fractionation and chemical characterization, it remains plausible that some active compounds in Elizabeth River porewater remain unidentified.
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影响因子：
3.8
作者：
Incardona, John P.;Linbo, Tiffany L.;Scholz, Nathaniel L.
通讯作者：
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影响因子：
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