Influence of nitrogen fertilization on soil ammonia oxidizer and denitrifier abundance, microbial biomass, and enzyme activities in an alpine meadow
Influence of nitrogen fertilization on soil ammonia oxidizer and denitrifier abundance, microbial biomass, and enzyme activities in an alpine meadow
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施氮肥对高寒草甸土壤氨氧化菌和反硝化菌丰度、微生物量及酶活性的影响
DOI：
10.1007/s00374-013-0889-0
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发表时间：
2014-05
影响因子：
6.5
通讯作者：
Liang-Dong Guo
中科院分区：
文献类型：
--
作者：
Xiao-Fang Tian;Hang-Wei Hu;Qiong Ding;Ming-Hua Song;Xing-Liang Xu;Yong Zheng;Liang-Dong Guo
据预测，陆地生态系统将经历大气氮(N)沉积水平的增加，这可能导致植物群落组成的显著变化，并伴随着刺激土壤酸化。然而，关于氮沉降对青藏高原等高寒草原生态系统中地下微生物群落的影响，人们知之甚少。研究了连续两个植物生长季节土壤氮素转化微生物(经DNA提取和定量聚合酶链式反应测定)、土壤微生物量C(SMBC)和N(SMBN)以及土壤酶活性对不同形态(NH_4+-N、NO_3−-N和NH_4NO_3-N)和施氮量(分别表示为低氮和高氮)和施氮量(分别表示为低氮和高氮)的响应。影响氨氧化古生菌(AOA)丰度的是氮肥用量，而不是氮素形态。高氮量显着提高了氨氧化细菌(AOB)的丰度，不同氮素形态处理的AOB丰度不同。施氮量对土壤反硝化细菌的丰度没有显著影响。施氮量较高时，SMBC和SMBN显著降低，但不同氮素形态间差异不显著。尽管在植物生长后期检测到较高的尿素酶活性，但转化酶和碱性磷酸单酯酶的活性在不同的氮素改良剂和植物生长季节都保持不变。潜在硝化速率与AOB丰度呈显著正相关。这些结果表明，在青藏高原高寒草甸土壤氮素循环过程中，AOB对不同施氮量的反应更为敏感，在土壤氮素循环过程中的作用可能比AOA更为显著。
Terrestrial ecosystems are predicted to experience an increasing level of atmospheric nitrogen (N) deposition, which may cause significant shifts in plant community composition and concomitantly stimulate soil acidification. However, little is known concerning the effects of N deposition on belowground microbial communities in alpine grassland ecosystems such as on the Tibetan Plateau. This study examined the responses of soil N-transforming microbes (measured after DNA extraction and quantitative PCR), soil microbial biomass C (SMBC) and N (SMBN), and soil enzyme activities to different forms (NH4+-N, NO3−-N, and NH4NO3-N) and rates (1.5 and 7.5 g N m−2year−1, denoted as low and high N, respectively) of N fertilization (addition) in two successive plant growing seasons. The N rate, not N form, influenced the abundance of ammonia-oxidizing archaea (AOA). High N addition significantly increased ammonia-oxidizing bacteria (AOB) abundance which differed across different N form treatments. Nitrogen addition had no significant impact on the abundance of soil denitrifiers. The SMBC and SMBN were significantly decreased by high N additions, but no difference was found among different N forms. Despite higher urease activities being detected in the late plant growing season, the activities of invertase and alkaline phosphomonoesterase stayed unchanged irrespective of the different N amendments and plant growing season. Significant positive correlations were found between potential nitrification rates and AOB abundances. These results highlight that AOB seemed to respond more sensitively to different N fertilization and might have prominent roles in soil N cycling processes in this Tibetan Plateau alpine meadow than AOA.
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影响因子：
5.2
作者：
Holden SR;Treseder KK
通讯作者：
Treseder KK
影响因子：
9.7
作者：
SCHINNER, F;VONMERSI, W
通讯作者：
VONMERSI, W
DOI：
10.2136/sssaj1965.03615995002900060025x
发表时间：
1965-11
影响因子：
2.9
作者：
F. Watanabe;S. R. Olsen
通讯作者：
F. Watanabe;S. R. Olsen
影响因子：
6.5
作者：
D. Fischer;M. Uksa;W. Tischler;T. Kautz;U. Köpke;M. Schloter
通讯作者：
D. Fischer;M. Uksa;W. Tischler;T. Kautz;U. Köpke;M. Schloter
氮负荷水平影响半干旱温带草原土壤氨氧化原核生物的丰度和组成
影响因子：
3.6
作者：
Shen, Xin-Yi;Zhang, Li-Mei;He, Ji-Zheng
通讯作者：
He, Ji-Zheng