Effects of lipooligosaccharide inner core truncation on bile resistance and chick colonization by Campylobacter jejuni.
Effects of lipooligosaccharide inner core truncation on bile resistance and chick colonization by Campylobacter jejuni.
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DOI：
10.1371/journal.pone.0056900
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Akiba M
中科院分区：
文献类型：
--
作者：
Iwata T;Chiku K;Amano K;Kusumoto M;Ohnishi-Kameyama M;Ono H;Akiba M
空肠弯曲菌是世界范围内引起腹泻的最常见的细菌，鸡被认为是这种病原体的主要宿主。本研究探讨了连续截短C. jejuni对鸡胆汁抵抗力和肠道定植能力的影响。失活编码甘露庚糖合成酶或糖基转移酶的基因以产生同基因突变体。LOS核心寡糖的连续截短导致两个C.空肠菌株NCTC 11168和81-176对胆汁酸的影响。hldE、hldD或waaC的失活导致核心寡糖的严重截短，这大大增加了对胆汁酸的敏感性。两种野生型菌株在鸡肠道提取物中正常生长，而具有严重寡糖截短的突变体在接种后12 h未检测到。接种后24 h，这些突变体在无胆汁酸提取物中达到活菌计数。野生型菌株11-164在攻击感染后5天和该时间段后以>107 CFU/g存在于盲肠内容物中，而其hldD突变体在整个实验期间以<103 CFU/g存在。hldD突变体与野生型hldD等位基因的反式互补完全恢复了野生型菌株的体内定殖水平。具有较短LOS的突变体具有较高的疏水性。因此，LOS核心寡糖的长度影响C.空肠以及其定殖鸡肠的能力。
Campylobacter jejuni is the most common bacterium that causes diarrhea worldwide, and chickens are considered the main reservoir of this pathogen. This study investigated the effects of serial truncation of lipooligosaccharide (LOS), a major component of the outer membrane of C. jejuni, on its bile resistance and intestinal colonization ability in chickens. Genes encoding manno-heptose synthetases or glycosyltransferases were inactivated to generate isogenic mutants. Serial truncation of the LOS core oligosaccharide caused a stepwise increase in susceptibilities of two C. jejuni strains, NCTC 11168 and 81-176, to bile acids. Inactivation of hldE, hldD, or waaC caused severe truncation of the core oligosaccharide, which greatly increased the susceptibility to bile acids. Both wild-type strains grew normally in chicken intestinal extracts, whereas the mutants with severe oligosaccharide truncation were not detected 12 h after inoculation. These mutants attained viable bacterial counts in the bile acid-free extracts 24 h after inoculation. The wild-type strain 11-164 was present in the cecal contents at >107 CFU/g on 5 days after challenge infection and after this time period, whereas its hldD mutant was present at <103 CFU/g throughout the experimental period. Trans-complementation of the hldD mutant with the wild-type hldD allele completely restored the in vivo colonization level to that of the wild-type strain. Mutants with a shorter LOS had higher hydrophobicities. Thus, the length of the LOS core oligosaccharide affected the surface hydrophobicity and bile resistance of C. jejuni as well as its ability to colonize chicken intestines.
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影响因子：
11.8
作者：
Coker AO;Isokpehi RD;Thomas BN;Amisu KO;Obi CL
通讯作者：
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