Clostridium perfringens epsilon toxin increases the small intestinal permeability in mice and rats.
Clostridium perfringens epsilon toxin increases the small intestinal permeability in mice and rats.
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DOI：
10.1371/journal.pone.0007065
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发表时间：
2009-09-18
期刊：
影响因子：
3.7
通讯作者：
Fernandez Miyakawa ME
中科院分区：
文献类型：
--
作者：
Goldstein J;Morris WE;Loidl CF;Tironi-Farinati C;McClane BA;Uzal FA;Fernandez Miyakawa ME
Epsilon 毒素是一种由 B 型和 D 型产气荚膜梭菌产生的强效神经毒素，这是一种厌氧细菌，可引起反刍动物肠毒血症。在受影响的动物中，它通过损害内皮细胞引起肺和脑水肿，引起生理和形态变化。尽管人们认为它会损害肠道屏障，从而进入肠道脉管系统，但人们对这一过程背后的机制知之甚少。本研究表征了 epsilon 毒素对小鼠和大鼠小肠液体运输和生物电参数的影响。肠池和肠环测试，以及单次灌注测定和尤辛室的体外和离体分析，都与小鼠和大鼠小肠的组织学和超微结构分析结合使用，无论是否有产气荚膜梭菌ε毒素攻击。 Luminal epsilon 毒素诱导时间和浓度依赖性肠液积聚和跨上皮阻力下降。虽然没有观察到明显的组织学变化，但通过透射电子显微镜观察到粘膜紧密连接的开放以及固有层的细胞凋亡变化。这些结果表明产气荚膜梭菌ε毒素主要通过打开粘膜紧密连接、增加其对大分子的渗透性以及诱导肠固有层的进一步退行性改变来改变肠道通透性。
Epsilon toxin is a potent neurotoxin produced by Clostridium perfringens types B and D, an anaerobic bacterium that causes enterotoxaemia in ruminants. In the affected animal, it causes oedema of the lungs and brain by damaging the endothelial cells, inducing physiological and morphological changes. Although it is believed to compromise the intestinal barrier, thus entering the gut vasculature, little is known about the mechanism underlying this process. This study characterizes the effects of epsilon toxin on fluid transport and bioelectrical parameters in the small intestine of mice and rats. The enteropooling and the intestinal loop tests, together with the single-pass perfusion assay and in vitro and ex vivo analysis in Ussing's chamber, were all used in combination with histological and ultrastructural analysis of mice and rat small intestine, challenged with or without C. perfringens epsilon toxin. Luminal epsilon toxin induced a time and concentration dependent intestinal fluid accumulation and fall of the transepithelial resistance. Although no evident histological changes were observed, opening of the mucosa tight junction in combination with apoptotic changes in the lamina propria were seen with transmission electron microscopy. These results indicate that C. perfringens epsilon toxin alters the intestinal permeability, predominantly by opening the mucosa tight junction, increasing its permeability to macromolecules, and inducing further degenerative changes in the lamina propria of the bowel.
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影响因子：
2.8
作者：
Losada-Eaton, D. M.;Uzal, F. A.;Miyakawa, M. E. Fernandez
通讯作者：
Miyakawa, M. E. Fernandez
影响因子：
0.8
作者：
FINNIE, JW
通讯作者：
FINNIE, JW
影响因子：
2.6
作者：
Minami, J;Katayama, S;Okabe, A
通讯作者：
Okabe, A
影响因子：
3.1
作者：
EWE, K
通讯作者：
EWE, K
兔回肠细胞外分流途径的离子电导。分流对透壁钠转运和电势差的影响。
DOI：
10.1085/jgp.59.3.318
发表时间：
1972-03
期刊：
The Journal of general physiology
影响因子：
--
作者：
Frizzell RA;Schultz SG
通讯作者：
Schultz SG