Plasmonic nanobiosensors for detection of microRNA cancer biomarkers in clinical samples.
Plasmonic nanobiosensors for detection of microRNA cancer biomarkers in clinical samples.
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DOI：
10.1039/d0an00193g
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发表时间：
2020-07-07
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MicroRNA(MiRNA)在生物学过程中起着重要的调节作用，在食管腺癌(EAC)和Barrett‘s Ecoagus(BE)等多种疾病的早期检测中具有广阔的应用前景。在这里，我们展示了使用我们团队开发的被称为反向分子哨兵(IMS)纳米生物传感器的纳米光子学技术，通过表面增强拉曼散射(SERS)检测，直接检测从17例内窥镜组织活检中提取的RNA中的食道癌生物标记物miR-21。通过区分食道癌和Barrett‘s食道与正常组织，证明了这种无标记、均质生物传感器在癌症诊断中的潜力，具有显著的诊断准确性。这项工作建立了IMS纳米生物传感器在癌症诊断中的潜力，通过在临床样本中检测miRNA而不需要靶标扩增，验证了这种检测作为新诊断策略的一部分的潜力。将miRNA诊断技术与纳米光子学技术相结合，将导致实现通用分子分析工具的范式转变，该工具广泛适用于癌症研究和癌症检测。我们期待这项技术的进一步发展，作为组织病理学诊断的替代方法，用于未来的护理点检测，因为我们的方法在RNA分离后提供了快速获得的结果，允许及时治疗。均相IMS生物传感器可以通过检测临床样本中的microRNA生物标记物来区分食道癌和正常组织。
MicroRNA (miRNA) play an important role in the regulation of biological processes and have demonstrated great promise as biomarkers for the early detection of various diseases, including esophageal adenocarcinoma (EAC) and Barrett’s esophagus (BE), the premalignant metaplasia associated with EAC. Herein, we demonstrate the direct detection of the esophageal cancer biomarker, miR-21, in RNA extracted from 17 endoscopic tissue biopsies using a nanophotonics technology our group has developed, termed inverse molecular sentinel (iMS) nanobiosensor, with surface-enhanced Raman scattering (SERS) detection. The potential of this label-free, homogenous biosensor for cancer diagnosis without the need for target amplification was demonstrated by discriminating esophageal cancer and Barrett’s esophagus from normal tissue with notable diagnostic accuracy. This work establishes the potential of the iMS nanobiosensors for cancer diagnostics via miRNA detection within clinical samples without the need for target amplification, validating the potential of this assay as part of a new diagnostic strategy. Combining miRNA diagnostics with nanophotonics technology will result in a paradigm shift in achieving a general molecular analysis tool that has widespread applicability for cancer research as well as detection of cancer. We anticipate the further development of this technique for future use in point-of-care testing as an alternative to histopathological diagnosis as our method provides a quickly obtained result following RNA isolation, allowing for timely treatment. Homogenous iMS biosensor allows for discrimination of esophageal cancer from normal tissue via detection of microRNA biomarkers within clinical samples.
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