Histomorphological investigation of intrahepatic connective tissue for surgical anatomy based on modern computer imaging analysis.
Histomorphological investigation of intrahepatic connective tissue for surgical anatomy based on modern computer imaging analysis.
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DOI：
10.1002/jhbp.753
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发表时间：
2021-01
影响因子：
3
通讯作者：
Wada H
中科院分区：
文献类型：
--
作者：
Ikeda T;Okano S;Hashimoto N;Kimura K;Kudo K;Tsutsumi R;Sasaki S;Kawasaki J;Miyashita Y;Wada H
计算机辅助组织成像和分析技术用于阐明肝结缔组织的组织形态学结构，作为外科医生的实用指南。从5名尸检患者的肝脏标本中制备了约5000张组织学切片。进行三维（3D）重建并进行计算机成像分析。扫描电子显微镜也进行了肝脏标本。三维重建图像显示了肝内血管的走行形态及肝小叶与结缔组织的关系。肝被膜或门静脉蒂始终位于肝小叶周围。人工智能随机森林方法清楚地分割了肝细胞、I型胶原（CF）、III型胶原（RF）和其他细胞。根据CF和RF占用率，肝小叶、门管区和肝包膜被显著区分。将直接覆盖肝小叶且RF浓度高达87%的包膜暂时命名为固有肝包膜。肝固有韧带的存在与CF和RF的占领率不同。肝固有被膜和肝固有韧带可作为外科手术中划分夹层的重要标志。 亮点计算机辅助组织成像和分析技术用于阐明肝结缔组织的组织形态学结构。Ikeda和同事们将注意力集中在肝小叶周围的包膜上。在外科手术中，识别出的肝固有囊和韧带似乎是划分夹层的重要结构。
Computer‐assisted tissue imaging and analytical techniques were used to clarify the histomorphological structure of hepatic connective tissue as a practical guide for surgeons. Approximately 5000 histological slides were prepared from liver specimens of five autopsied patients. Three‐dimensional (3D) reconstruction was performed and subjected to computer imaging analysis. Scanning electron microscopy was also performed on the liver specimens. The 3D reconstructed images revealed the running form of the vasculature and the relationship between the hepatic lobule and connective tissue. The hepatic capsule or portal pedicle was consistently located at the periphery of the hepatic lobules. An artificial intelligence random forest approach clearly segmented hepatic cells, type I collagen (CF), type III collagen (RF), and other cells. The hepatic lobule, portal region, and hepatic capsule were significantly distinguished based on CF and RF occupancy. The capsule directly covering the liver lobule with an RF concentration up to 87% was provisionally named the proper hepatic capsule. The existence of a proper hepatic ligament with distinct occupation rates of CF and RF was also suggested. The identified proper hepatic capsule and ligament can be important markers for demarcating the dissecting layer during surgical procedures. Highlight Computer‐assisted tissue imaging and analytical techniques were used to clarify the histomorphological structure of hepatic connective tissue. Ikeda and colleagues focused on the capsule surrounding the hepatic lobule itself. The identified proper hepatic capsule and ligament appear to be an important structure for demarcating the dissecting layer during surgical procedures.
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DOI：
10.1073/pnas.73.2.539
发表时间：
1976-01-01
影响因子：
11.1
作者：
ROJKIND, M;MARTINEZPALOMO, A
通讯作者：
MARTINEZPALOMO, A
影响因子：
2.9
作者：
SEYER, JM;MAINARDI, C;KANG, AH
通讯作者：
KANG, AH
影响因子：
5.8
作者：
Arganda-Carreras, Ignacio;Kaynig, Verena;Seung, H. Sebastian
通讯作者：
Seung, H. Sebastian
影响因子：
2.8
作者：
Lormand, Charline;Zellmer, Georg F.;Moebis, Anja
通讯作者：
Moebis, Anja
影响因子：
2
作者：
Nishimura, Shotaro;Sagara, Ayano;Tabata, Shoji
通讯作者：
Tabata, Shoji